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血管生成素1(ANGPT1)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)

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×
本試劑盒運(yùn)用雙抗體夾心 ELISA 法定量測(cè)定人血清、血漿或其它相關(guān)生物液體中 ANGPT1 含量。
編號(hào):SEA008Hu
參數(shù)品牌:云克隆
產(chǎn)品參數(shù)
品牌:云克隆
型號(hào):SEA008Hu
起訂量:1
規(guī)格::96T
價(jià)格::¥2988
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在線客服
產(chǎn)品詳情
  • 編號(hào)SEA008Hu

  • 物種Homo sapiens (Human,人) 相同的名稱,不同的物種。

  • 實(shí)驗(yàn)方法雙抗夾心

  • 反應(yīng)時(shí)長(zhǎng)3h

  • 檢測(cè)范圍0.24-15ng/mL

  • 靈敏度最小可檢測(cè)劑量小于等于0.11ng/mL.

  • 樣本類型Serum, plasma, tissue homogenates and other biological fluids

  • 下載英文說明書   中文說明書

  • 規(guī)格48T96T 96T*5 96T*10 96T*100

  • 價(jià)格¥ 2988



特異性

本試劑盒用于檢測(cè)血管生成素1(ANGPT1),經(jīng)檢測(cè)與其它相似物質(zhì)無明顯交叉反應(yīng)。
由于受到技術(shù)及樣本來源的限制,不可能完成對(duì)所有相關(guān)或相似物質(zhì)交叉反應(yīng)檢測(cè),因此本試劑盒有可能與未經(jīng)檢測(cè)的其它物質(zhì)有交叉反應(yīng)。

回收率

分別于定值血清及血漿樣本中加入一定量的血管生成素1(ANGPT1)(加標(biāo)樣品),重復(fù)測(cè)定并計(jì)算其均值,回收率為測(cè)定值與理論值的比率。

樣本回收率范圍(%)平均回收率(%)
serum(n=5)83-9590
EDTA plasma(n=5)86-9490
heparin plasma(n=5)81-9488

精密度

精密度用樣品測(cè)定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差:取同批次試劑盒對(duì)低、中、高值定值樣本進(jìn)行定量檢測(cè),每份樣本連續(xù)測(cè)定20 次,分別計(jì)算不同濃度樣本的平均值及SD值。
批間差:選取3個(gè)不同批次的試劑盒分別對(duì)低、中、高值定值樣本進(jìn)行定量測(cè)定,每個(gè)樣本使用同一試劑盒重復(fù)測(cè)定8次,分別計(jì)算不同濃度樣本的平均值及SD值。
批內(nèi)差: CV<10%
批間差: CV<12%

線性

在定值血清及血漿樣本內(nèi)加入適量的血管生成素1(ANGPT1),并倍比稀釋成1:2,1:4,1:8,1:16的待測(cè)樣本,線性范圍即為稀釋后樣本中血管生成素1(ANGPT1)含量的測(cè)定值與理論值的比率。

樣本1:21:41:81:16
serum(n=5)79-92%82-102%78-103%83-97%
EDTA plasma(n=5)87-99%90-101%80-93%88-103%
heparin plasma(n=5)85-97%85-99%83-91%82-96%

穩(wěn)定性

經(jīng)測(cè)定,試劑盒在有效期內(nèi)按推薦溫度保存,其活性降低率小于5%。
為減小外部因素對(duì)試劑盒破壞前后檢測(cè)值的影響,實(shí)驗(yàn)室的環(huán)境條件需盡量保持一致,尤其是實(shí)驗(yàn)室內(nèi)溫度、濕度及溫育條件。其次由同一實(shí)驗(yàn)員來進(jìn)行操作可減少人為誤差。

實(shí)驗(yàn)流程

1. 實(shí)驗(yàn)前標(biāo)準(zhǔn)品、試劑及樣本的準(zhǔn)備;
2. 加樣(標(biāo)準(zhǔn)品及樣本)100μL,37°C孵育1小時(shí);
3. 吸棄,加檢測(cè)溶液A100μL,37°C孵育1小時(shí);
4. 洗板3次;
5. 加檢測(cè)溶液B100μL,37°C孵育30分鐘;
6. 洗板5次;
7. 加TMB底物90μL,37°C孵育10-20分鐘;
8. 加終止液50μL,立即450nm讀數(shù)。

實(shí)驗(yàn)原理

將血管生成素1(ANGPT1)抗體包被于96孔微孔板中,制成固相載體,向微孔中分別加入標(biāo)準(zhǔn)品或標(biāo)本,其中的血管生成素1(ANGPT1)與連接于固相載體上的抗體結(jié)合,然后加入生物素化的血管生成素1(ANGPT1)抗體,將未結(jié)合的生物素化抗體洗凈后,加入HRP標(biāo)記的親和素,再次徹底洗滌后加入TMB底物顯色。TMB在過氧化物酶的催化下轉(zhuǎn)化成藍(lán)色,并在酸的作用下轉(zhuǎn)化成最終的黃色。顏色的深淺和樣品中的血管生成素1(ANGPT1)呈正相關(guān)。用酶標(biāo)儀在450nm波長(zhǎng)下測(cè)定吸光度(O.D.值),計(jì)算樣品濃度。

參考文獻(xiàn)

雜志參考文獻(xiàn)
TheriogenologyEvaluation of Changes in Doppler Ultrasonography Indices and Levels of Maternal Serum Angiogenic Factors throughout Pregnancy in Ewes [Science: Article]
J Atheroscler ThrombHigh Mobility Group Box 1 Promotes Angiogenesis from Bone Marrow-derived Endothelial Progenitor Cells after Myocardial Infarction [PubMed: 25735431]
Archives of Biological SciencesDiabetes-induced renal failure is associated with tissue inflammation and neutrophil gelatinase-associated lipocalin: Effects of resveratrol [publication:299492265_Diabetes-induced_renal_failu]
Am J Transl ResAngiopoietin-1 facilitates recovery of hematopoiesis in radiated mice [Pubmed:27347310]
Clinical researchIncreased plasma concentrations of interleukin 35 in patients with coronary artery disease [Journal:-19]
Turkish Journal of Medicl SciencesA new angiogenesis prognostic index with VEGFA, PlGF, and angiopoietin 1 predicts survival in patients with advanced gastric cancer [pubmed:28425270]
Sci Rep. Flunarizine suppresses endothelial Angiopoietin-2 in a calcium - dependent fashion in sepsis. [pubmed:28276491]
PeerJ The ratio of serum Angiopoietin-1 to Angiopoietin-2 in patients with cervical cancer is a valuable diagnostic and prognostic biomarker [pubmed:28584715]
Molecular&cellular proteomicsPrediction of Response to Sorafenib in Hepatocellular Carcinoma: A Marker Panel by Multiple Reaction Monitoring-Mass Spectrometry [pubmed:28550167]
Tumor BiologyCervical cancer cell–derived angiopoietins promote tumor progression [pubmed:28720059]
Free Patents onlineRHEUMATOID ARTHRITIS RELATED BIOMARKER [20170350884]
AnesthesiologyVasculotide, an angiopoietin-1 mimetic, restores microcirculatory perfusion and microvascular leakage and decreases fluid resuscitation requirements in … [Pubmed:28968277]
British Journal of AnaesthesiaVasculotide, an angiopoietin-1 mimetic, reduces pulmonary vascular leakage and preserves microcirculatory perfusion during cardiopulmonary bypass in rats [10.1016:j.bja.2018.05.049]
The Journal of Biological ChemistryToll-like receptor 2 deficiency hyperactivates the FoxO1 transcription factor and induces aging-associated cardiac dysfunction in mice [Pubmed:29929978]

Clinical Significance of Nectin-4 Expression in Metastasis and Angiogenesis for Tumor Relapse [Doi: 10.4143/crt.2018.214]
BIOLOGY OF REPRODUCTIONProstaglandin-mediated effects in early canine corpus luteum: In vivo effects on vascular and immune factors [Pubmed: 30929911]
Scientific ReportsAssociation between Upper-airway Surgery and Ameliorative Risk Markers of endothelial function in obstructive Sleep Apnea [Pubmed: 31882827]
Biochem Biophys Res CommunEffects of interleukin-6 and tumor necrosis factor-α on the expression of angiogenic and collagenolytic factors in premature and mature adipocytes [Pubmed: 32800538]
J Nutr BiochemEffects of protein malnutrition on haematopoietic regulatory activity of bone marrow mesenchymal stem cells [33705953]
Intensive Care Med ExpThe effect of targeting Tie2 on hemorrhagic shock-induced renal perfusion disturbances in rats [33997943]
Gynecol EndocrinolPotential of thrombospondin-1 in treatment of polycystic ovary syndrome rat model: a preliminary study [34282706]


血管生成素1(ANGPT1)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)
血管生成素1(ANGPT1)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)

血管生成素1(ANGPT1)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)

分享到微信

×
本試劑盒運(yùn)用雙抗體夾心 ELISA 法定量測(cè)定人血清、血漿或其它相關(guān)生物液體中 ANGPT1 含量。
編號(hào):SEA008Hu
品牌:云克隆
型號(hào):SEA008Hu
起訂量:1
規(guī)格::96T
價(jià)格::¥2988
15906629305
在線客服
產(chǎn)品詳情
  • 編號(hào)SEA008Hu

  • 物種Homo sapiens (Human,人) 相同的名稱,不同的物種。

  • 實(shí)驗(yàn)方法雙抗夾心

  • 反應(yīng)時(shí)長(zhǎng)3h

  • 檢測(cè)范圍0.24-15ng/mL

  • 靈敏度最小可檢測(cè)劑量小于等于0.11ng/mL.

  • 樣本類型Serum, plasma, tissue homogenates and other biological fluids

  • 下載英文說明書   中文說明書

  • 規(guī)格48T96T 96T*5 96T*10 96T*100

  • 價(jià)格¥ 2988



特異性

本試劑盒用于檢測(cè)血管生成素1(ANGPT1),經(jīng)檢測(cè)與其它相似物質(zhì)無明顯交叉反應(yīng)。
由于受到技術(shù)及樣本來源的限制,不可能完成對(duì)所有相關(guān)或相似物質(zhì)交叉反應(yīng)檢測(cè),因此本試劑盒有可能與未經(jīng)檢測(cè)的其它物質(zhì)有交叉反應(yīng)。

回收率

分別于定值血清及血漿樣本中加入一定量的血管生成素1(ANGPT1)(加標(biāo)樣品),重復(fù)測(cè)定并計(jì)算其均值,回收率為測(cè)定值與理論值的比率。

樣本回收率范圍(%)平均回收率(%)
serum(n=5)83-9590
EDTA plasma(n=5)86-9490
heparin plasma(n=5)81-9488

精密度

精密度用樣品測(cè)定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差:取同批次試劑盒對(duì)低、中、高值定值樣本進(jìn)行定量檢測(cè),每份樣本連續(xù)測(cè)定20 次,分別計(jì)算不同濃度樣本的平均值及SD值。
批間差:選取3個(gè)不同批次的試劑盒分別對(duì)低、中、高值定值樣本進(jìn)行定量測(cè)定,每個(gè)樣本使用同一試劑盒重復(fù)測(cè)定8次,分別計(jì)算不同濃度樣本的平均值及SD值。
批內(nèi)差: CV<10%
批間差: CV<12%

線性

在定值血清及血漿樣本內(nèi)加入適量的血管生成素1(ANGPT1),并倍比稀釋成1:2,1:4,1:8,1:16的待測(cè)樣本,線性范圍即為稀釋后樣本中血管生成素1(ANGPT1)含量的測(cè)定值與理論值的比率。

樣本1:21:41:81:16
serum(n=5)79-92%82-102%78-103%83-97%
EDTA plasma(n=5)87-99%90-101%80-93%88-103%
heparin plasma(n=5)85-97%85-99%83-91%82-96%

穩(wěn)定性

經(jīng)測(cè)定,試劑盒在有效期內(nèi)按推薦溫度保存,其活性降低率小于5%。
為減小外部因素對(duì)試劑盒破壞前后檢測(cè)值的影響,實(shí)驗(yàn)室的環(huán)境條件需盡量保持一致,尤其是實(shí)驗(yàn)室內(nèi)溫度、濕度及溫育條件。其次由同一實(shí)驗(yàn)員來進(jìn)行操作可減少人為誤差。

實(shí)驗(yàn)流程

1. 實(shí)驗(yàn)前標(biāo)準(zhǔn)品、試劑及樣本的準(zhǔn)備;
2. 加樣(標(biāo)準(zhǔn)品及樣本)100μL,37°C孵育1小時(shí);
3. 吸棄,加檢測(cè)溶液A100μL,37°C孵育1小時(shí);
4. 洗板3次;
5. 加檢測(cè)溶液B100μL,37°C孵育30分鐘;
6. 洗板5次;
7. 加TMB底物90μL,37°C孵育10-20分鐘;
8. 加終止液50μL,立即450nm讀數(shù)。

實(shí)驗(yàn)原理

將血管生成素1(ANGPT1)抗體包被于96孔微孔板中,制成固相載體,向微孔中分別加入標(biāo)準(zhǔn)品或標(biāo)本,其中的血管生成素1(ANGPT1)與連接于固相載體上的抗體結(jié)合,然后加入生物素化的血管生成素1(ANGPT1)抗體,將未結(jié)合的生物素化抗體洗凈后,加入HRP標(biāo)記的親和素,再次徹底洗滌后加入TMB底物顯色。TMB在過氧化物酶的催化下轉(zhuǎn)化成藍(lán)色,并在酸的作用下轉(zhuǎn)化成最終的黃色。顏色的深淺和樣品中的血管生成素1(ANGPT1)呈正相關(guān)。用酶標(biāo)儀在450nm波長(zhǎng)下測(cè)定吸光度(O.D.值),計(jì)算樣品濃度。

參考文獻(xiàn)

雜志參考文獻(xiàn)
TheriogenologyEvaluation of Changes in Doppler Ultrasonography Indices and Levels of Maternal Serum Angiogenic Factors throughout Pregnancy in Ewes [Science: Article]
J Atheroscler ThrombHigh Mobility Group Box 1 Promotes Angiogenesis from Bone Marrow-derived Endothelial Progenitor Cells after Myocardial Infarction [PubMed: 25735431]
Archives of Biological SciencesDiabetes-induced renal failure is associated with tissue inflammation and neutrophil gelatinase-associated lipocalin: Effects of resveratrol [publication:299492265_Diabetes-induced_renal_failu]
Am J Transl ResAngiopoietin-1 facilitates recovery of hematopoiesis in radiated mice [Pubmed:27347310]
Clinical researchIncreased plasma concentrations of interleukin 35 in patients with coronary artery disease [Journal:-19]
Turkish Journal of Medicl SciencesA new angiogenesis prognostic index with VEGFA, PlGF, and angiopoietin 1 predicts survival in patients with advanced gastric cancer [pubmed:28425270]
Sci Rep. Flunarizine suppresses endothelial Angiopoietin-2 in a calcium - dependent fashion in sepsis. [pubmed:28276491]
PeerJ The ratio of serum Angiopoietin-1 to Angiopoietin-2 in patients with cervical cancer is a valuable diagnostic and prognostic biomarker [pubmed:28584715]
Molecular&cellular proteomicsPrediction of Response to Sorafenib in Hepatocellular Carcinoma: A Marker Panel by Multiple Reaction Monitoring-Mass Spectrometry [pubmed:28550167]
Tumor BiologyCervical cancer cell–derived angiopoietins promote tumor progression [pubmed:28720059]
Free Patents onlineRHEUMATOID ARTHRITIS RELATED BIOMARKER [20170350884]
AnesthesiologyVasculotide, an angiopoietin-1 mimetic, restores microcirculatory perfusion and microvascular leakage and decreases fluid resuscitation requirements in … [Pubmed:28968277]
British Journal of AnaesthesiaVasculotide, an angiopoietin-1 mimetic, reduces pulmonary vascular leakage and preserves microcirculatory perfusion during cardiopulmonary bypass in rats [10.1016:j.bja.2018.05.049]
The Journal of Biological ChemistryToll-like receptor 2 deficiency hyperactivates the FoxO1 transcription factor and induces aging-associated cardiac dysfunction in mice [Pubmed:29929978]

Clinical Significance of Nectin-4 Expression in Metastasis and Angiogenesis for Tumor Relapse [Doi: 10.4143/crt.2018.214]
BIOLOGY OF REPRODUCTIONProstaglandin-mediated effects in early canine corpus luteum: In vivo effects on vascular and immune factors [Pubmed: 30929911]
Scientific ReportsAssociation between Upper-airway Surgery and Ameliorative Risk Markers of endothelial function in obstructive Sleep Apnea [Pubmed: 31882827]
Biochem Biophys Res CommunEffects of interleukin-6 and tumor necrosis factor-α on the expression of angiogenic and collagenolytic factors in premature and mature adipocytes [Pubmed: 32800538]
J Nutr BiochemEffects of protein malnutrition on haematopoietic regulatory activity of bone marrow mesenchymal stem cells [33705953]
Intensive Care Med ExpThe effect of targeting Tie2 on hemorrhagic shock-induced renal perfusion disturbances in rats [33997943]
Gynecol EndocrinolPotential of thrombospondin-1 in treatment of polycystic ovary syndrome rat model: a preliminary study [34282706]


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