Tunicamycin (衣霉素)

Tunicamycin 是一種同源核苷抗生su的混合物, 可抑制 N-糖基化并阻斷 GlcNAc 磷酸轉(zhuǎn)移酶 (GPT)。Tunicamycin 引起細(xì)胞內(nèi)質(zhì)網(wǎng) (ER) 中未折疊蛋白的積累并誘導(dǎo) ER 應(yīng)激，并導(dǎo)致 DNA 合成受阻和 G1 期細(xì)胞周期停滯。Tunicamycin 可抑制革蘭氏陽(yáng)性細(xì)菌，酵母，真菌和病毒，并具有抗癌活性。Tunicamycin 增加宮頸癌細(xì)胞中外泌體的釋放。

貨號(hào)：HY-A0098
CAS：11089-65-9

參數(shù)品牌：MCE

產(chǎn)品參數(shù)

品牌：MCE

型號(hào)：HY-A0098

起訂量：1

規(guī)格:：2mg

價(jià)格:：￥1925

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Tunicamycin (Synonyms: 衣霉素)

Tunicamycin 是一種同源核苷抗生素的混合物, 可抑制 N-糖基化并阻斷 GlcNAc 磷酸轉(zhuǎn)移酶 (GPT)。Tunicamycin 引起細(xì)胞內(nèi)質(zhì)網(wǎng) (ER) 中未折疊蛋白的積累并誘導(dǎo) ER 應(yīng)激，并導(dǎo)致 DNA 合成受阻和 G1 期細(xì)胞周期停滯。Tunicamycin 可抑制革蘭氏陽(yáng)性細(xì)菌，酵母，真菌和病毒，并具有抗癌活性。Tunicamycin 增加宮頸癌細(xì)胞中外泌體的釋放。

生物活性

Tunicamycin is a mixture of homologous nucleoside antibiotic that inhibits N-linked glycosylation and blocks GlcNAc phosphotransferase (GPT). Tunicamycin causes accumulation of unfolded proteins in cell endoplasmic reticulum (ER) and induces ER stress, and causes blocking of DNA synthesis and cell cycle arrest in G1 phase. Tunicamycin inhibits gram-positive bacteria, yeasts, fungi, and viruses and has anti-cancer activity^[1]^[2]^[3].Tunicamycin increases exosome release in cervical cancer cells^[4].

體外研究(In Vitro)

Tunicamycin (2 μg/mL; 24 hours; CD44+/CD24- and original MCF7 cells) treatment increases the spliced XBP-1, ATF6 nuclear translocation level and CHOP protein expression in CD44+/CD24- and original MCF7 cells^[1].
Tunicamycin-induced ER stress suppresses CD44+/CD24- phenotype cell subpopulation and in vitro invasion and accelerates tumorosphore formation. Under effect of Tunicamycin, the results show that inhibited invasion, increased cell death, suppressed proliferation and reduced migration in the CD44+/CD24- and CD44+/CD24- rich MCF7 cell culture^[1]

Western Blot Analysis

Cell Line:	CD44+/CD24- and original MCF7 cells^[1]
Concentration:	2 μg/mL
Incubation Time:	24 hours
Result:	Increased level of spliced XBP-1, ATF6 nuclear translocation and CHOP protein expression are detected in CD44+/CD24- and original MCF7 cells.

體內(nèi)研究(In Vivo)

Tunicamycin (0.1 mg/kg or 0.5 mg/kg) treatment dramatically suppresses tumor growth in the CD133^+/- MHCC97L cells xenograft model (BALB/c (nu/nu) mice)^[2]

分子量：844.94 (n=10)

Formula：C₃₉H₆₄N₄O₁₆

CAS 號(hào)：11089-65-9

中文名稱：衣霉素；鏈病毒菌素

運(yùn)輸條件：Room temperature in continental US; may vary elsewhere.

儲(chǔ)存方式：

Powder	-20°C	3 years
In solvent	-80°C	6 months
	-20°C	1 month

溶解性數(shù)據(jù)

DMSO : 40 mg/mL (Need ultrasonic)

H₂O : < 0.1 mg/mL (insoluble)

In Vivo:

以下溶解方案都請(qǐng)先按照 In Vitro 方式配制澄清的儲(chǔ)備液，再依次添加助溶劑：

——為保證實(shí)驗(yàn)結(jié)果的可靠性，澄清的儲(chǔ)備液可以根據(jù)儲(chǔ)存條件，適當(dāng)保存；體內(nèi)實(shí)驗(yàn)的工作液，建議您現(xiàn)用現(xiàn)配，當(dāng)天使用；以下溶劑前顯示的百
分比是指該溶劑在您配制終溶液中的體積占比；如在配制過(guò)程中出現(xiàn)沉淀、析出現(xiàn)象，可以通過(guò)加熱和/或超聲的方式助溶

1.

請(qǐng)依序添加每種溶劑： 10% DMSO 40% PEG300 5% Tween-80 45% saline
Solubility: ≥ 2 mg/mL (Infinity mM); Clear solution
2.

請(qǐng)依序添加每種溶劑： 10% DMSO 90% (20% SBE-β-CD in saline)
Solubility: ≥ 2 mg/mL (Infinity mM); Clear solution
3.

請(qǐng)依序添加每種溶劑： 10% DMSO 90% corn oil
Solubility: ≥ 2 mg/mL (Infinity mM); Clear solution

參考文獻(xiàn)

[1]. Hsu JL, et al. Tunicamycin induces resistance to camptothecin and etoposide in human hepatocellular carcinoma cells: role of cell-cycle arrest and GRP78. Naunyn Schmiedebergs Arch Pharmacol. 2009 Nov;380(5):373-82.

[2]. Hou H, et al. DPAGT1/Akt/ABCG2 pathway in mouse Xenograft models of human hepatocellular carcinoma. Mol Cancer Ther. 2013 Dec;12(12):2874-84.

[3]. Han C, et al. Endoplasmic reticulum stress inhibits cell cycle progression via induction of p27 in melanoma cells. Cell Signal. 2013 Jan;25(1):144-9.

[4]. Kathleen M McAndrews, et al. Mechanisms associated with biogenesis of exosomes in cancer. Mol Cancer. 2019 Mar 30;18(1):52.

Tunicamycin (衣霉素)

Tunicamycin 是一種同源核苷抗生su的混合物, 可抑制 N-糖基化并阻斷 GlcNAc 磷酸轉(zhuǎn)移酶 (GPT)。Tunicamycin 引起細(xì)胞內(nèi)質(zhì)網(wǎng) (ER) 中未折疊蛋白的積累并誘導(dǎo) ER 應(yīng)激，并導(dǎo)致 DNA 合成受阻和 G1 期細(xì)胞周期停滯。Tunicamycin 可抑制革蘭氏陽(yáng)性細(xì)菌，酵母，真菌和病毒，并具有抗癌活性。Tunicamycin 增加宮頸癌細(xì)胞中外泌體的釋放。

貨號(hào)：HY-A0098
CAS：11089-65-9

品牌：MCE

型號(hào)：HY-A0098

起訂量：1

規(guī)格:：2mg

價(jià)格:：￥1925

15906629305

在線客服

客服一

產(chǎn)品詳情

Tunicamycin (Synonyms: 衣霉素)

Tunicamycin 是一種同源核苷抗生素的混合物, 可抑制 N-糖基化并阻斷 GlcNAc 磷酸轉(zhuǎn)移酶 (GPT)。Tunicamycin 引起細(xì)胞內(nèi)質(zhì)網(wǎng) (ER) 中未折疊蛋白的積累并誘導(dǎo) ER 應(yīng)激，并導(dǎo)致 DNA 合成受阻和 G1 期細(xì)胞周期停滯。Tunicamycin 可抑制革蘭氏陽(yáng)性細(xì)菌，酵母，真菌和病毒，并具有抗癌活性。Tunicamycin 增加宮頸癌細(xì)胞中外泌體的釋放。

生物活性

Tunicamycin is a mixture of homologous nucleoside antibiotic that inhibits N-linked glycosylation and blocks GlcNAc phosphotransferase (GPT). Tunicamycin causes accumulation of unfolded proteins in cell endoplasmic reticulum (ER) and induces ER stress, and causes blocking of DNA synthesis and cell cycle arrest in G1 phase. Tunicamycin inhibits gram-positive bacteria, yeasts, fungi, and viruses and has anti-cancer activity^[1]^[2]^[3].Tunicamycin increases exosome release in cervical cancer cells^[4].

體外研究(In Vitro)

Tunicamycin (2 μg/mL; 24 hours; CD44+/CD24- and original MCF7 cells) treatment increases the spliced XBP-1, ATF6 nuclear translocation level and CHOP protein expression in CD44+/CD24- and original MCF7 cells^[1].
Tunicamycin-induced ER stress suppresses CD44+/CD24- phenotype cell subpopulation and in vitro invasion and accelerates tumorosphore formation. Under effect of Tunicamycin, the results show that inhibited invasion, increased cell death, suppressed proliferation and reduced migration in the CD44+/CD24- and CD44+/CD24- rich MCF7 cell culture^[1]

Western Blot Analysis

Cell Line:	CD44+/CD24- and original MCF7 cells^[1]
Concentration:	2 μg/mL
Incubation Time:	24 hours
Result:	Increased level of spliced XBP-1, ATF6 nuclear translocation and CHOP protein expression are detected in CD44+/CD24- and original MCF7 cells.

體內(nèi)研究(In Vivo)

Tunicamycin (0.1 mg/kg or 0.5 mg/kg) treatment dramatically suppresses tumor growth in the CD133^+/- MHCC97L cells xenograft model (BALB/c (nu/nu) mice)^[2]

分子量：844.94 (n=10)

Formula：C₃₉H₆₄N₄O₁₆

CAS 號(hào)：11089-65-9

中文名稱：衣霉素；鏈病毒菌素

運(yùn)輸條件：Room temperature in continental US; may vary elsewhere.

儲(chǔ)存方式：

Powder	-20°C	3 years
In solvent	-80°C	6 months
	-20°C	1 month

溶解性數(shù)據(jù)

DMSO : 40 mg/mL (Need ultrasonic)

H₂O : < 0.1 mg/mL (insoluble)

In Vivo:

以下溶解方案都請(qǐng)先按照 In Vitro 方式配制澄清的儲(chǔ)備液，再依次添加助溶劑：

——為保證實(shí)驗(yàn)結(jié)果的可靠性，澄清的儲(chǔ)備液可以根據(jù)儲(chǔ)存條件，適當(dāng)保存；體內(nèi)實(shí)驗(yàn)的工作液，建議您現(xiàn)用現(xiàn)配，當(dāng)天使用；以下溶劑前顯示的百
分比是指該溶劑在您配制終溶液中的體積占比；如在配制過(guò)程中出現(xiàn)沉淀、析出現(xiàn)象，可以通過(guò)加熱和/或超聲的方式助溶

1.

請(qǐng)依序添加每種溶劑： 10% DMSO 40% PEG300 5% Tween-80 45% saline
Solubility: ≥ 2 mg/mL (Infinity mM); Clear solution
2.

請(qǐng)依序添加每種溶劑： 10% DMSO 90% (20% SBE-β-CD in saline)
Solubility: ≥ 2 mg/mL (Infinity mM); Clear solution
3.

請(qǐng)依序添加每種溶劑： 10% DMSO 90% corn oil
Solubility: ≥ 2 mg/mL (Infinity mM); Clear solution

參考文獻(xiàn)

[1]. Hsu JL, et al. Tunicamycin induces resistance to camptothecin and etoposide in human hepatocellular carcinoma cells: role of cell-cycle arrest and GRP78. Naunyn Schmiedebergs Arch Pharmacol. 2009 Nov;380(5):373-82.

[2]. Hou H, et al. DPAGT1/Akt/ABCG2 pathway in mouse Xenograft models of human hepatocellular carcinoma. Mol Cancer Ther. 2013 Dec;12(12):2874-84.

[3]. Han C, et al. Endoplasmic reticulum stress inhibits cell cycle progression via induction of p27 in melanoma cells. Cell Signal. 2013 Jan;25(1):144-9.

[4]. Kathleen M McAndrews, et al. Mechanisms associated with biogenesis of exosomes in cancer. Mol Cancer. 2019 Mar 30;18(1):52.

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Tunicamycin (Synonyms: 衣霉素)

Tunicamycin (衣霉素)

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Tunicamycin (Synonyms: 衣霉素)