EGF 蛋白, Human

EGF 蛋白, Human是一種重要的表皮生長(zhǎng)因子，能夠刺激表皮細(xì)胞增殖，可應(yīng)用于傷口愈合的研究。

種屬：Human

表達(dá)系統(tǒng)：E. coli

蛋白編號(hào)：P01133 (N971-R1023)

基因 ID：1950

中文名：重組人表皮生長(zhǎng)因子

同用名：rHuEGF; Pro-epidermal growth factor; Urogastrone

氨基酸序列：MNSDSECPLSHDGYCLHDGVCMYIEALDKYACNCVVGYIGERCQYRDLKWWELR

分子量：Approximately 6.2 kDa

生物活性：The ED₅₀ is <0.2 ng/mL as measured by murine BALB/c 3T3 cells, corresponding to a specific activity of 5.0 × 10⁶ units/mg.

性狀：Lyophilized powder

組分：Lyophilized after extensive dialysis against 10mM Phosphate buffer, pH 7.0, 200 mM NaCl buffer.

內(nèi)毒素含量：<0.2 EU/μg, determined by LAL method.

復(fù)溶方法：It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

保存條件 & 期限：Stored at -20°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

運(yùn)輸條件：Room temperature in continental US; may vary elsewhere.

描述：Recombinant Human Epidermal Growth Factor is chemically identical to the natural material, exhibits full biological activity and is ued in wound healing applications^[1]. Recombinant Human Epidermal Growth Factor (rhEGF) stimulates proliferation of the fibroblast BALB/c3T3 cell line. Recombinant Human Epidermal Growth Factor released from hydrogels keeps its bioactivity, induces EGF receptor expression, causes proliferating cell nuclear antigen and shows therapeutic potential in enhancing diabetic wound healing^[2].

參考文獻(xiàn)

[1]. Wong WR, et al. Applications, and efficient large-scale production, of recombinant human epidermal growth factor. Biotechnol Genet Eng Rev. 2001;18:51-71.

[2]. Lao G, et al. Controlled release of epidermal growth factor from hydrogels accelerates wound healing in diabetic rats. J Am Podiatr Med Assoc. 2012 Mar-Apr;102(2):89-98.