取消
清空記錄
歷史記錄
清空記錄
歷史記錄
產(chǎn)品中心
生物活性
5-BrdU (BrdU) is a nucleoside analog that competes with thymidine for incorporation into DNA. 5-BrdU is commonly used in the detection of proliferating cells.
體外研究(In Vitro)
Bromodeoxyuridine induces a progressive, dose-responsive suppression of cancer cell line and cancer stem cell population expansion RG2 rat glioma cells. In H9 cells and BJ fibroblasts, bromodeoxyuridine alters the cell cycle profile[1]. BrdU is stably integrated into the DNA, and thus can be used in assessment of cell proliferation and other cell procession[2].
體內(nèi)研究(In Vivo)
Bromodeoxyuridine (300 mg/kg, i.p. or 0.8 mg/mL, p.o.) significantly slows tumor progression in rat glioma RG2 tumor model[1].
分子量:307.10
Formula:C9H11BrN2O5
CAS 號:59-14-3
中文名稱:5-溴脫氧尿嘧啶核苷
運(yùn)輸條件:Room temperature in continental US; may vary elsewhere.
儲存方式:
4°C, protect from light
*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
溶解性數(shù)據(jù)
DMSO : 250 mg/mL (814.07 mM; Need ultrasonic)
H2O : 2 mg/mL (6.51 mM; ultrasonic and warming and heat to 60°C)
濃度溶劑體積質(zhì)量 | 1 mg | 5 mg | 10 mg |
---|
1 mM | 3.2563 mL | 16.2813 mL | 32.5627 mL |
5 mM | 0.6513 mL | 3.2563 mL | 6.5125 mL |
10 mM | 0.3256 mL | 1.6281 mL | 3.2563 mL |
請根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲備液;一旦配成溶液,請分裝保存,避免反復(fù)凍融造成的產(chǎn)品失效。
儲備液的保存方式和期限:-80°C, 6 months; -20°C, 1 month (protect from light)。-80°C 儲存時(shí),請?jiān)?6 個(gè)月內(nèi)使用,-20°C 儲存時(shí),請?jiān)?1 個(gè)月內(nèi)使用。
以下溶解方案都請先按照 In Vitro 方式配制澄清的儲備液,再依次添加助溶劑:
——為保證實(shí)驗(yàn)結(jié)果的可靠性,澄清的儲備液可以根據(jù)儲存條件,適當(dāng)保存;體內(nèi)實(shí)驗(yàn)的工作液,建議您現(xiàn)用現(xiàn)配,當(dāng)天使用; 以下溶劑前顯示的百
分比是指該溶劑在您配制終溶液中的體積占比;如在配制過程中出現(xiàn)沉淀、析出現(xiàn)象,可以通過加熱和/或超聲的方式助溶
請依序添加每種溶劑: PBS
Solubility: 16.25 mg/mL (52.91 mM); Clear solution; Need ultrasonic
請依序添加每種溶劑: 10% DMSO 90% corn oil
參考文獻(xiàn)
生物活性
5-BrdU (BrdU) is a nucleoside analog that competes with thymidine for incorporation into DNA. 5-BrdU is commonly used in the detection of proliferating cells.
體外研究(In Vitro)
Bromodeoxyuridine induces a progressive, dose-responsive suppression of cancer cell line and cancer stem cell population expansion RG2 rat glioma cells. In H9 cells and BJ fibroblasts, bromodeoxyuridine alters the cell cycle profile[1]. BrdU is stably integrated into the DNA, and thus can be used in assessment of cell proliferation and other cell procession[2].
體內(nèi)研究(In Vivo)
Bromodeoxyuridine (300 mg/kg, i.p. or 0.8 mg/mL, p.o.) significantly slows tumor progression in rat glioma RG2 tumor model[1].
分子量:307.10
Formula:C9H11BrN2O5
CAS 號:59-14-3
中文名稱:5-溴脫氧尿嘧啶核苷
運(yùn)輸條件:Room temperature in continental US; may vary elsewhere.
儲存方式:
4°C, protect from light
*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
溶解性數(shù)據(jù)
DMSO : 250 mg/mL (814.07 mM; Need ultrasonic)
H2O : 2 mg/mL (6.51 mM; ultrasonic and warming and heat to 60°C)
濃度溶劑體積質(zhì)量 | 1 mg | 5 mg | 10 mg |
---|
1 mM | 3.2563 mL | 16.2813 mL | 32.5627 mL |
5 mM | 0.6513 mL | 3.2563 mL | 6.5125 mL |
10 mM | 0.3256 mL | 1.6281 mL | 3.2563 mL |
請根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲備液;一旦配成溶液,請分裝保存,避免反復(fù)凍融造成的產(chǎn)品失效。
儲備液的保存方式和期限:-80°C, 6 months; -20°C, 1 month (protect from light)。-80°C 儲存時(shí),請?jiān)?6 個(gè)月內(nèi)使用,-20°C 儲存時(shí),請?jiān)?1 個(gè)月內(nèi)使用。
以下溶解方案都請先按照 In Vitro 方式配制澄清的儲備液,再依次添加助溶劑:
——為保證實(shí)驗(yàn)結(jié)果的可靠性,澄清的儲備液可以根據(jù)儲存條件,適當(dāng)保存;體內(nèi)實(shí)驗(yàn)的工作液,建議您現(xiàn)用現(xiàn)配,當(dāng)天使用; 以下溶劑前顯示的百
分比是指該溶劑在您配制終溶液中的體積占比;如在配制過程中出現(xiàn)沉淀、析出現(xiàn)象,可以通過加熱和/或超聲的方式助溶
請依序添加每種溶劑: PBS
Solubility: 16.25 mg/mL (52.91 mM); Clear solution; Need ultrasonic
請依序添加每種溶劑: 10% DMSO 90% corn oil
參考文獻(xiàn)