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5-BrdU (5-溴脫氧尿嘧啶核苷)

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5-BrdU (BrdU) 是一種核苷類似物,可與胸苷競爭摻入 DNA。5-BrdU 通常用于檢測增殖細(xì)胞。

貨號:HY-15910
CAS:59-14-3
參數(shù)品牌:MCE
產(chǎn)品參數(shù)
品牌:MCE
型號:HY-15910
起訂量:1
規(guī)格::100mg
價(jià)格::¥450
我知道了
在線客服
產(chǎn)品詳情

5-BrdU (Synonyms: 5-溴脫氧尿嘧啶核苷)


生物活性

5-BrdU (BrdU) is a nucleoside analog that competes with thymidine for incorporation into DNA. 5-BrdU is commonly used in the detection of proliferating cells.


體外研究(In Vitro)

Bromodeoxyuridine induces a progressive, dose-responsive suppression of cancer cell line and cancer stem cell population expansion RG2 rat glioma cells. In H9 cells and BJ fibroblasts, bromodeoxyuridine alters the cell cycle profile[1]. BrdU is stably integrated into the DNA, and thus can be used in assessment of cell proliferation and other cell procession[2].


體內(nèi)研究(In Vivo)

Bromodeoxyuridine (300 mg/kg, i.p. or 0.8 mg/mL, p.o.) significantly slows tumor progression in rat glioma RG2 tumor model[1].


分子量:307.10


Formula:C9H11BrN2O5


CAS 號:59-14-3


中文名稱:5-溴脫氧尿嘧啶核苷


運(yùn)輸條件:Room temperature in continental US; may vary elsewhere.


儲存方式:

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)


溶解性數(shù)據(jù)


In Vitro: 

DMSO : 250 mg/mL (814.07 mM; Need ultrasonic)

H2O : 2 mg/mL (6.51 mM; ultrasonic and warming and heat to 60°C)

配制儲備液
濃度溶劑體積質(zhì)量1 mg5 mg10 mg
1 mM3.2563 mL16.2813 mL32.5627 mL
5 mM0.6513 mL3.2563 mL6.5125 mL
10 mM0.3256 mL1.6281 mL3.2563 mL
*

請根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲備液;一旦配成溶液,請分裝保存,避免反復(fù)凍融造成的產(chǎn)品失效。
儲備液的保存方式和期限:-80°C, 6 months; -20°C, 1 month (protect from light)。-80°C 儲存時(shí),請?jiān)?6 個(gè)月內(nèi)使用,-20°C 儲存時(shí),請?jiān)?1 個(gè)月內(nèi)使用。

In Vivo:

以下溶解方案都請先按照 In Vitro 方式配制澄清的儲備液,再依次添加助溶劑:

——為保證實(shí)驗(yàn)結(jié)果的可靠性,澄清的儲備液可以根據(jù)儲存條件,適當(dāng)保存;體內(nèi)實(shí)驗(yàn)的工作液,建議您現(xiàn)用現(xiàn)配,當(dāng)天使用; 以下溶劑前顯示的百
分比是指該溶劑在您配制終溶液中的體積占比;如在配制過程中出現(xiàn)沉淀、析出現(xiàn)象,可以通過加熱和/或超聲的方式助溶

  • 1.


    請依序添加每種溶劑: PBS

    Solubility: 16.25 mg/mL (52.91 mM); Clear solution; Need ultrasonic


  • 2.


    請依序添加每種溶劑: 10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 2.08 mg/mL (6.77 mM); Clear solution


  • 3.


    請依序添加每種溶劑: 10% DMSO    90% (20% SBE-β-CD in saline)

    Solubility: ≥ 2.08 mg/mL (6.77 mM); Clear solution


  • 4.


    請依序添加每種溶劑: 10% DMSO    90% corn oil



參考文獻(xiàn)

[1]. Levkoff LH, et al. Bromodeoxyuridine inhibits cancer cell proliferation in vitro and in vivo. Neoplasia. 2008 Aug;10(8):804-16.

[2]. Rothaeusler K, et al. Assessment of cell proliferation by 5-bromodeoxyuridine (BrdU) labeling for multicolor flow cytometry. Curr Protoc Cytom. 2007 Apr;Chapter 7:Unit7.31











5-BrdU (5-溴脫氧尿嘧啶核苷)

5-BrdU (5-溴脫氧尿嘧啶核苷)

分享到微信

×
5-BrdU (BrdU) 是一種核苷類似物,可與胸苷競爭摻入 DNA。5-BrdU 通常用于檢測增殖細(xì)胞。

貨號:HY-15910
CAS:59-14-3
品牌:MCE
型號:HY-15910
起訂量:1
規(guī)格::100mg
價(jià)格::¥450
15906629305
在線客服
產(chǎn)品詳情

5-BrdU (Synonyms: 5-溴脫氧尿嘧啶核苷)


生物活性

5-BrdU (BrdU) is a nucleoside analog that competes with thymidine for incorporation into DNA. 5-BrdU is commonly used in the detection of proliferating cells.


體外研究(In Vitro)

Bromodeoxyuridine induces a progressive, dose-responsive suppression of cancer cell line and cancer stem cell population expansion RG2 rat glioma cells. In H9 cells and BJ fibroblasts, bromodeoxyuridine alters the cell cycle profile[1]. BrdU is stably integrated into the DNA, and thus can be used in assessment of cell proliferation and other cell procession[2].


體內(nèi)研究(In Vivo)

Bromodeoxyuridine (300 mg/kg, i.p. or 0.8 mg/mL, p.o.) significantly slows tumor progression in rat glioma RG2 tumor model[1].


分子量:307.10


Formula:C9H11BrN2O5


CAS 號:59-14-3


中文名稱:5-溴脫氧尿嘧啶核苷


運(yùn)輸條件:Room temperature in continental US; may vary elsewhere.


儲存方式:

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)


溶解性數(shù)據(jù)


In Vitro: 

DMSO : 250 mg/mL (814.07 mM; Need ultrasonic)

H2O : 2 mg/mL (6.51 mM; ultrasonic and warming and heat to 60°C)

配制儲備液
濃度溶劑體積質(zhì)量1 mg5 mg10 mg
1 mM3.2563 mL16.2813 mL32.5627 mL
5 mM0.6513 mL3.2563 mL6.5125 mL
10 mM0.3256 mL1.6281 mL3.2563 mL
*

請根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲備液;一旦配成溶液,請分裝保存,避免反復(fù)凍融造成的產(chǎn)品失效。
儲備液的保存方式和期限:-80°C, 6 months; -20°C, 1 month (protect from light)。-80°C 儲存時(shí),請?jiān)?6 個(gè)月內(nèi)使用,-20°C 儲存時(shí),請?jiān)?1 個(gè)月內(nèi)使用。

In Vivo:

以下溶解方案都請先按照 In Vitro 方式配制澄清的儲備液,再依次添加助溶劑:

——為保證實(shí)驗(yàn)結(jié)果的可靠性,澄清的儲備液可以根據(jù)儲存條件,適當(dāng)保存;體內(nèi)實(shí)驗(yàn)的工作液,建議您現(xiàn)用現(xiàn)配,當(dāng)天使用; 以下溶劑前顯示的百
分比是指該溶劑在您配制終溶液中的體積占比;如在配制過程中出現(xiàn)沉淀、析出現(xiàn)象,可以通過加熱和/或超聲的方式助溶

  • 1.


    請依序添加每種溶劑: PBS

    Solubility: 16.25 mg/mL (52.91 mM); Clear solution; Need ultrasonic


  • 2.


    請依序添加每種溶劑: 10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 2.08 mg/mL (6.77 mM); Clear solution


  • 3.


    請依序添加每種溶劑: 10% DMSO    90% (20% SBE-β-CD in saline)

    Solubility: ≥ 2.08 mg/mL (6.77 mM); Clear solution


  • 4.


    請依序添加每種溶劑: 10% DMSO    90% corn oil



參考文獻(xiàn)

[1]. Levkoff LH, et al. Bromodeoxyuridine inhibits cancer cell proliferation in vitro and in vivo. Neoplasia. 2008 Aug;10(8):804-16.

[2]. Rothaeusler K, et al. Assessment of cell proliferation by 5-bromodeoxyuridine (BrdU) labeling for multicolor flow cytometry. Curr Protoc Cytom. 2007 Apr;Chapter 7:Unit7.31











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