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RN10-EASYspin 酵母RNA快速提取試劑盒
貨號# | 規(guī)格 | 價格 |
RN1001 | 50 次 | 1050元(帶lytic Enzyme) |
產(chǎn)品介紹:
酵母細胞經(jīng)lyticase處理去除細胞壁后,獨特的裂解液/β-巰基乙醇迅速裂解細胞和滅活細胞RNA酶,然后用乙醇調(diào)節(jié)結(jié)合條件后,RNA選擇性吸附于離心柱內(nèi)硅基質(zhì)膜, 再通過一系列快速的漂洗-離心的步驟, 去蛋白液和漂洗液將細胞代謝物,蛋白等雜質(zhì)去除, z后低鹽的RNase free H20將純凈RNA從硅基質(zhì)膜上洗脫。
產(chǎn)品特點:
1.離心吸附柱內(nèi)硅基質(zhì)膜全部采用進口世界有名公司特制吸附膜,柱與柱之間吸附量差異極小,可重復性好??朔藝a(chǎn)試劑盒膜質(zhì)量不穩(wěn)定的弊端。
2.不需要使用有毒的苯酚,氯仿等試劑,也不需要乙醇沉淀等步驟。
3.快速,簡捷,單個樣品操作一般可在20分鐘內(nèi)完成。
4.多次柱漂洗確保高純度,OD260/OD280典型的比值達1.9~2.0,基本無DNA殘留,可用于RT-PCR,Northern-blot和各種實驗。
使用該產(chǎn)品發(fā)表的部分文章:http://www.aidlab.cn/news-show.asp?id=185
BDSF inhibits Candida albicans adherence to urinary catheters. Microbial Pathogenesis 2013, 64: 33-38
Expression analysis of VfDGAT2 in various tissues of the tung tree and in transgenic yeast.Genet. Mol. Res.2013, 12 (4): 6554-6564
Effects of tung oilseed FAD2 and DGAT2 genes on unsaturated fatty acid accumulation in Rhodotorula glutinis and Arabidopsis thaliana. Mol Genet Genomics 2015, 290: 1605–1613
Production of 3-Hydroxypropionic Acid via the Propionyl-CoA Pathway Using Recombinant Escherichia coli Strains. PLOS ONE | DOI:10.1371/journal.pone.0156286 May 26, 2016
The FKS family genes cause changes in cell wall morphology resulted in regulation of anti-autolytic ability in Saccharomyces cerevisiae. Bioresource Technology 2018, 249: 49-56
Differential expression analysis of the SRB1 gene in fluconazole-resistant and susceptible strains of Candida albicans.The Journal of Antibiotics 2020, 73: 309–313
RN10-EASYspin 酵母RNA快速提取試劑盒
貨號# | 規(guī)格 | 價格 |
RN1001 | 50 次 | 1050元(帶lytic Enzyme) |
產(chǎn)品介紹:
酵母細胞經(jīng)lyticase處理去除細胞壁后,獨特的裂解液/β-巰基乙醇迅速裂解細胞和滅活細胞RNA酶,然后用乙醇調(diào)節(jié)結(jié)合條件后,RNA選擇性吸附于離心柱內(nèi)硅基質(zhì)膜, 再通過一系列快速的漂洗-離心的步驟, 去蛋白液和漂洗液將細胞代謝物,蛋白等雜質(zhì)去除, z后低鹽的RNase free H20將純凈RNA從硅基質(zhì)膜上洗脫。
產(chǎn)品特點:
1.離心吸附柱內(nèi)硅基質(zhì)膜全部采用進口世界有名公司特制吸附膜,柱與柱之間吸附量差異極小,可重復性好??朔藝a(chǎn)試劑盒膜質(zhì)量不穩(wěn)定的弊端。
2.不需要使用有毒的苯酚,氯仿等試劑,也不需要乙醇沉淀等步驟。
3.快速,簡捷,單個樣品操作一般可在20分鐘內(nèi)完成。
4.多次柱漂洗確保高純度,OD260/OD280典型的比值達1.9~2.0,基本無DNA殘留,可用于RT-PCR,Northern-blot和各種實驗。
使用該產(chǎn)品發(fā)表的部分文章:http://www.aidlab.cn/news-show.asp?id=185
BDSF inhibits Candida albicans adherence to urinary catheters. Microbial Pathogenesis 2013, 64: 33-38
Expression analysis of VfDGAT2 in various tissues of the tung tree and in transgenic yeast.Genet. Mol. Res.2013, 12 (4): 6554-6564
Effects of tung oilseed FAD2 and DGAT2 genes on unsaturated fatty acid accumulation in Rhodotorula glutinis and Arabidopsis thaliana. Mol Genet Genomics 2015, 290: 1605–1613
Production of 3-Hydroxypropionic Acid via the Propionyl-CoA Pathway Using Recombinant Escherichia coli Strains. PLOS ONE | DOI:10.1371/journal.pone.0156286 May 26, 2016
The FKS family genes cause changes in cell wall morphology resulted in regulation of anti-autolytic ability in Saccharomyces cerevisiae. Bioresource Technology 2018, 249: 49-56
Differential expression analysis of the SRB1 gene in fluconazole-resistant and susceptible strains of Candida albicans.The Journal of Antibiotics 2020, 73: 309–313