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RN53-EASYspin Plus 多糖/多酚/復(fù)雜植物RNA快速提取試劑盒
產(chǎn)品貨號(hào) | 產(chǎn)品名稱 | 規(guī)格 | 報(bào)價(jià) | 特價(jià) |
RN5301 | EASYspin Plus 多糖/多酚/復(fù)雜植物RNA快速提取試劑盒 | 50次 | 1580 | 1265 |
產(chǎn)品介紹:
本公司推出EASYspin無苯酚、氯仿RNA快速提取技術(shù)基礎(chǔ)上,又研發(fā)成功基因組DNA清chu柱技術(shù)可以有效清chugDNA殘留,得到的RNA一般不需要DNase消化,可用于反轉(zhuǎn)錄PCR、熒光定量PCR等實(shí)驗(yàn)。獨(dú)特的裂解液迅速裂解細(xì)胞和滅活細(xì)胞RNA酶, 離心沉淀去除多糖多酚和次級(jí)代謝產(chǎn)物,然后裂解混合物用乙醇調(diào)節(jié)RNA結(jié)合吸附到基因組DNA清chu柱,然后RNA被選擇性洗脫濾過, 吸附在基因組DNA清chu柱上的殘留DNA無法洗脫連同柱子一起丟棄從而清chu掉DNA。濾過的RNA用乙醇調(diào)節(jié)結(jié)合條件后,RNA在高離序鹽狀態(tài)下選擇性吸附于離心柱內(nèi)硅基質(zhì)膜,再通過一系列快速的漂洗-離心的步驟,去蛋白液和漂洗液將細(xì)胞代謝物,蛋白等雜質(zhì)去除, Z后低鹽的RNase free H20將純凈RNA從硅基質(zhì)膜上洗脫。
產(chǎn)品特點(diǎn):
1.完全不使用有毒的苯酚,氯仿等試劑,也不需要乙醇沉淀等步驟。
2.簡(jiǎn)捷,單個(gè)樣品操作一般可在25分鐘內(nèi)完成,世界上簡(jiǎn)單快速的試劑盒。
3.成功研發(fā)基因組DNA清chu柱技術(shù)可以有效清chugDNA殘留,得到的RNA一般不需要DNase消化,可用于反轉(zhuǎn)錄PCR、熒光定量PCR等實(shí)驗(yàn)。
4.世界先進(jìn)的適應(yīng)性極其廣,可以提取包括復(fù)雜中草藥如石斛/丹參/雪蓮/人參、復(fù)雜淀粉種子如水稻/小麥/玉米種子、復(fù)雜果實(shí)如葡萄/藍(lán)莓/草莓/西瓜果實(shí)、復(fù)雜抗逆植物如冬青/松針/沙棘/胡楊、復(fù)雜花卉月季/玫瑰/梅/牡丹花、復(fù)雜多糖植物紫菜/仙人掌/蘆薈水稻種子等數(shù)百種國(guó)內(nèi)外試劑盒提取失敗的樣品,使用EASYspin系列植物RNA提取試劑盒累計(jì)發(fā)表文章超過200篇。詳細(xì)樣品列表請(qǐng)參考公司主頁(yè)產(chǎn)品介紹或者找我們索取200多篇發(fā)表文章原文。
5.多次柱漂洗確保高純度,OD260/OD280典型的比值達(dá)1.9~2.2,無DNA殘留,可用于熒光定量PCR、RT-PCR、芯片、二代測(cè)序、Northern-blot等各種實(shí)驗(yàn)。
使用EASYspin/EASYspin Plus系列植物RNA提取試劑盒已經(jīng)有大量文章發(fā)表,請(qǐng)聯(lián)系我們索取發(fā)表的文章和包括北京林業(yè)大學(xué),北京農(nóng)學(xué)院,中國(guó)農(nóng)業(yè)大學(xué),農(nóng)林科學(xué)院果樹研究所,林業(yè)所,中國(guó)農(nóng)科院等大學(xué)和研究院所大量使用案例。
1. 桃果實(shí)、花、根、葉:Isolation, characterisation and phylogenetic analysis of resistance gene analogues in a wild species of peach (Prunus kansuensis).Canadian Journal of Plant Science, 2011, 91(6): 961-970
2. 櫻桃花、葉、顎等各部位:Over-expression of the PaAP1 gene from sweet cherry (Prunus avium L.) causes early floweri.Journal of Plant Physiology, 2012,Available online 1 December 2012
3. 洋蔥根、莖、蕾、葉、雌雄蕊等各部位:Cloning and Expression Analysis of A Putative B Class MADS-box Gene of AcPI in Onion. Scientia Agricultura Sinica, 2012, 45(23):4759-4769
4. 蕪菁:Isolation and Functional Characterisation of the Genes Encoding Δ8-Sphingolipid Desaturase from Brassica rapa. Journal of Genetics and Genomics Volume 39, Issue 1, January 2012, Pages 47–59
5. 蕪 菁 1 :EXPRESSION, DIVERGENCE AND EVOLUTION OF THE CALEOSIN GENE FAMILY IN BRASSICA
6. 番茄葉:Effect of Low Temperature Stress on the Expression of ProDH Gene and the Activities of the Proline Dehydrogenase in Leaves of Tomato Seedling. Chinese Agricultural Science Bulletin 2012,28(10):132-135
7. 梔子葉:Isolation of High Quality Total RNA from Gardenia jasminoides Eills.Chinese Agricultural Science Bulletin.2012, 28(27):194-198
8. 油桐果實(shí):Cui Qinqin, Han Xiaojiao, Chen Yicun, Zhan Zhiyong, Lin Liyuan, Wang Yangdong. Isolation and Expression Characteristics of Biotin Carboxyl Carrier Protein Coding Gene(VfBCCP) from Vernicia fordii.SCIENTIA SILVAE SINICAE. 2012, 48(8): Available online August
9. 油桐果實(shí)1:Selection of Reliable Reference Genes for Gene Expression Studies Using Real-Time PCR in Tung Tree during Seed Development. PLoS ONE, 2012, 7(8): e43084
10. 紫菜:Molecular cloning and expression analysis of ribosomal protein S7 gene from Porphyra haitanensis. JOURNAL OF FISHERIES OF CHINA, 2011, 35(12):1814-1821
11. 石斛:Molecular characterization of a mitogen-activated protein kinase gene DoMPK
12. 石斛1:ESTs Analysis Reveals Putative Genes Involved in Symbiotic Seed Germination in Dendrobium officinale. Symbiotic Germination Genes in D. officinale. August 2013 | Volume 8 | Issue 8 | e72705
13. 大豆:RNA-seq Analysis Reveals Ethylene-Mediated Reproductive Organ Development and Abscission in Soybean(Glycine max L. Merr.). Plant Mol Biol Rep, 2012, published online: 4 Dec, 2012
14. 大豆1:Construction of ethylene regulatory network based on the phytohormones related gene transcriptome profiling and prediction of transcription factor activities in soybean. Acta Physiol Plant, 2012, published online: 12 Dec, 2012
15. 紅花玉蘭:Expression Analysis of MAwuAG in Different Organs and Developmental Stages of Magnolia wufengensis. Chinese Bulletin of Botany, 2013, 48 (2): 1–5
16. 毛桃:Cloning and Phylogeny Analysis of PpAP2 Floral Homologous Genes in Peach. Chinese Agricultural Science Bulletin, 2013, 29(7): 99-104
17. 五倍子:Cloning and characterisation of a phenylalanine ammonia-lyase gene from Rhus chinensis. Plant Cell Rep, 2013, published online:15 March, 2013
18. :五倍子1:Cloning, characterization and expression of chalcone synthase from medicinal plant Rhus chinensis.J. Plant Biochem. Biotechnol. DOI 10.1007/s13562-013-0231-9
19. 青杄 :cDNA Cloning and Bioinformatic Analysis of the sPPa1 Gene form Picea wilsonii. Plant Science Journal, 2012, 30(40): 394-401
20. 青杄 1:cDNA Cloning and Bioinformatic Analysis of PsbO Gene from Picea wilsonii.Life Science Research, 2012, 16(3): 201-206
21. 青杄 2:Cloning and Tissue Expression Analysis of PwPSAF in Picea wilsonii. SCIENTIA SILVAE SINICAE. Vol. 49,No. 10, Oct. 2013.
22. 洋蔥:Molecular Cloning and Transcriptional Analysis of the Putative AGAMOUS Homolog AcAG in Onion (Allium cepa. Plant Mol Biol Rep, DOI 10.1007/s11105-013-0607-y
23. 木瓜:XsFAD2 gene encodes the enzyme responsible for the high linoleic acid content in oil accumulated in Xanthoceras sorbifolia seeds. JOURNAL ARTICLE.
24. 木瓜1:Two novel diacylglycerol acyltransferase genes from Xanthoceras 2 sorbifolia are responsible for its seed oil content. GENE-38688; No. of pages: 9;
25. 柑橘:Efficient auto-excision of a selectable marker gene from transgenic citrus by combining the Cre/loxP system and ipt selection. Plant Cell Rep, DOI 10.1007/s00299-013-1470-x
26. 柑橘1:Expression Analysis of Three Phloem-specific Promoters in Transgenic Poncirus trifoliata. Acta Horticulturae Sinica. 2014, 41(1): 1–8.
27. 柑橘2: Activation of three pathogen-inducible promoters in transgenic citrus (Citrus sinensis Osbeck) after Xanthomonas axonopodis pv. citri infection and wounding. Plant Cell Tiss Organ Cult. DOI 10.1007/s11240-013-0423-y.
28. 茶梅花瓣:Comparison and Analysis of Methods of Extracting Total RNA from Petals of Camellia sasanqua. Chinese Agricultural Science Bulletin.2013,29(28):129-133.
29. 梔子:Isolation of High Quality Total RNA fromGardenia jasminoides Eills. Chinese Agricultural Science Bulletin. 2012, 28(27):194-198
30. 丹參:Genome-wide analysis and molecular dissection of the SPL gene family in Salvia miltiorrhiza. 2014 Jan;56(1):38-50. doi: 10.1111/jipb.12111. Epub 2013 Nov 20.
31. 牡丹:Transcriptome Comparison Reveals Key Candidate Genes Responsible for the Unusual Reblooming Trait in Tree Peonies. Genes Responsible for Reblooming in Tree Peonies. November 2013 | Volume 8 | Issue 11 | e79996
32. 東南景天:Role of sulfur assimilation pathway in cadmium hyperaccumulation by Sedum alfredii Hance. Ecotoxicology and Environmental Safety. Volume 100, February 2014, Pages 159–165.
33. 山蒼子:Identification of appropriate reference genes for normalizing transcript expression by quantitative real?time PCR in Litsea cubeba. TECHNICAL NOTE. Mol Genet Genomics (2013) 288:727–737, DOI 10.1007/s00438-013-0785-1
34. 木本植物:Heterologous gene silencing induced by tobacco rattle virus (TRV) is efficient for pursuing functional genomics studies in woody plants. ORIGINAL PAPER. Plant Cell Tiss Organ Cult, DOI 10.1007/s11240-013-0393-0
35. 棉花:Analysis of sea-island cotton and upland cotton in response to Verticillium dahliae infection by RNA sequencing. Sun et al. BMC Genomics 2013, 14:852 /1471-2164/14/852.
36. 桃子:Biochemical changes and defence responses during the development of peach gummosis caused by Lasiodiplodia theobromae. Eur J Plant Pathol (2014) 138:195–207, DOI 10.1007/s10658-013-0322-4.
37. 桃子1:Carbohydrate metabolism changes in Prunus persica gummosis infected with Lasiodiplodia theobromae. Phytopathology "First Look" paper ? http://dx.doi.org/10.1094/PHYTO-
38. 海棠:The Malus crabapple transcription factor McMYB10 regulatesanthocyanin biosynthesis during petal coloration. Scientia Horticulturae 166 (2014) 42–49.
39. 海藻:A rapid and sensitive method for field detection of Prorocentrum donghaiense using reverse transcription-coupled loop-mediated isothermal amplification. Harmful Algae 29 (2013) 31–39.
40. 油茶:Establish a cDNA-AFLP Technology System in Camellia oleifera. Molecular Plant Breeding, 2013, Vol.11, No.5, 611-616.
41. 亞洲百合:Transcriptomic analysis of Asiatic lily in the process of vernalization via RNA-seq. Mol Biol Rep. DOI 10.1007/s11033-014-3250-2.
42. 毛泡桐:Dynamic expression of novel and conserved microRNAs and their targets in diploid and tetraploid of Paulownia tomentosa. Biochimie xxx (2014) 1e10.
43. 人參:Cloning and Sequence Analysis Squalene Epoxidase Gene in Panax gin-seng. Journal of
44. 雪蓮:Cloning and Sequence Analysis of rbcs Gene from Sasussured involucrdta Kar. et Kir. Chinese Agricultural Science Bulletin 2014, 30(15): 261-267
45. 柑橘3:Secreted Expression of Cecropin B Gene Enhances Resistance to Xanthomonas axonopodis pv. citri in Transgenic Citrus sinensis‘Tarocco’ Acta Horticulturae Sinica 2014, 41(3): 417–428 http: // www. ahs. ac. cn
46. 菊花:Stem apex detoxification culture markedly improved severalphysiological characters of chrysanthemum ‘YUTAI’. Plant Cell Tiss Organ Cult 2014, DOI 10.1007/s11240-014-0541-1
47. 蕎麥和擬南芥:Ectopic expression of FaesAP3, a Fagopyrum esculentum (Polygonaceae) AP3 orthologous gene rescues stamen development in an Arabidopsis ap3 mutant. Gene 2014, 550(2): 200–206
48. 油松:Differential expression of SLOW WALKER2 homologue in ovules of female sterile mutant and fertile clone of Pinus tabulaeformis. Russian Journal of Developmental Biology 2014, 45(2): 78-84
49. 玫瑰花:Precise spatio-temporal modulation of ACC synthase by MPK6 cascade mediates the response of rose flowers to rehydration. The Plant Journal 2014, 79(6): 941–950
50. 棉花和擬南芥:Functional characterization of GhAKT1, a novel Shaker-like K+ channel gene involved in K+ uptake from cotton (Gossypium hirsutum). Gene 2014, 545(1): 61–71
51. 棉花和擬南芥1:Upland Cotton Gene GhFPF1 Confers Promotion of Flowering Time and Shade-Avoidance Responses in Arabidopsis thaliana. PLoS ONE 2014, 9(3): e91869. doi:10.1371/journal.pone.0091869
52. 白楊:Poplar GATA transcription factor PdGNC is capable of regulating chloroplast ultrastructure, photosynthesis, and vegetative growth in Arabidopsis under varying nitrogen levels. Plant Cell Tiss Organ Cult 2014, DOI 10.1007/s11240-014-0536-y
53. 毛果楊:Molecular characterization of the SPL gene family in Populus trichocarpa. BMC Plant Biology 2014, 14: 131
54. 葛根:Molecular cloning and characterization of an isoflavone 7-O-glucosyltransferase from Pueraria lobata. Plant Cell Reports 2014, 33(7), 1173–1185
55. 百合:Cloning and Expression Analysis of Actin Gene(lilyActin)from Lily. Acta Horticulturae Sinica 2013, 40(7): 1318–1326
56. 百合1: Vernalization of Oriental hybrid lily ‘Sorbonne’: changes in physiology metabolic activity and molecular mechanism. Molecular Biology Reports 2014, DOI 10.1007/s11033-014-3545-3
57. 黃鵪菜:Transcriptome Sequencing and De Novo Analysis of Youngia japonica Using the Illumina Platform. PLoS ONE 2014, 9(3): e90636. doi:10.1371/journal.pone.0090636
58. 棉花1:Gibberellin Overproduction Promotes Sucrose Synthase Expression and Secondary Cell Wall Deposition in Cotton Fibers. PLoS ONE 2014, 9(5): e96537. doi:10.1371/journal.pone.0096537
59. 蘋果:Low Medium pH Value Enhances Anthocyanin Accumulation in Malus Crabapple Leaves. PLoS ONE 2014, 9(6): e97904. doi:10.1371/journal.pone.0097904
RN53-EASYspin Plus 多糖/多酚/復(fù)雜植物RNA快速提取試劑盒
產(chǎn)品貨號(hào) | 產(chǎn)品名稱 | 規(guī)格 | 報(bào)價(jià) | 特價(jià) |
RN5301 | EASYspin Plus 多糖/多酚/復(fù)雜植物RNA快速提取試劑盒 | 50次 | 1580 | 1265 |
產(chǎn)品介紹:
本公司推出EASYspin無苯酚、氯仿RNA快速提取技術(shù)基礎(chǔ)上,又研發(fā)成功基因組DNA清chu柱技術(shù)可以有效清chugDNA殘留,得到的RNA一般不需要DNase消化,可用于反轉(zhuǎn)錄PCR、熒光定量PCR等實(shí)驗(yàn)。獨(dú)特的裂解液迅速裂解細(xì)胞和滅活細(xì)胞RNA酶, 離心沉淀去除多糖多酚和次級(jí)代謝產(chǎn)物,然后裂解混合物用乙醇調(diào)節(jié)RNA結(jié)合吸附到基因組DNA清chu柱,然后RNA被選擇性洗脫濾過, 吸附在基因組DNA清chu柱上的殘留DNA無法洗脫連同柱子一起丟棄從而清chu掉DNA。濾過的RNA用乙醇調(diào)節(jié)結(jié)合條件后,RNA在高離序鹽狀態(tài)下選擇性吸附于離心柱內(nèi)硅基質(zhì)膜,再通過一系列快速的漂洗-離心的步驟,去蛋白液和漂洗液將細(xì)胞代謝物,蛋白等雜質(zhì)去除, Z后低鹽的RNase free H20將純凈RNA從硅基質(zhì)膜上洗脫。
產(chǎn)品特點(diǎn):
1.完全不使用有毒的苯酚,氯仿等試劑,也不需要乙醇沉淀等步驟。
2.簡(jiǎn)捷,單個(gè)樣品操作一般可在25分鐘內(nèi)完成,世界上簡(jiǎn)單快速的試劑盒。
3.成功研發(fā)基因組DNA清chu柱技術(shù)可以有效清chugDNA殘留,得到的RNA一般不需要DNase消化,可用于反轉(zhuǎn)錄PCR、熒光定量PCR等實(shí)驗(yàn)。
4.世界先進(jìn)的適應(yīng)性極其廣,可以提取包括復(fù)雜中草藥如石斛/丹參/雪蓮/人參、復(fù)雜淀粉種子如水稻/小麥/玉米種子、復(fù)雜果實(shí)如葡萄/藍(lán)莓/草莓/西瓜果實(shí)、復(fù)雜抗逆植物如冬青/松針/沙棘/胡楊、復(fù)雜花卉月季/玫瑰/梅/牡丹花、復(fù)雜多糖植物紫菜/仙人掌/蘆薈水稻種子等數(shù)百種國(guó)內(nèi)外試劑盒提取失敗的樣品,使用EASYspin系列植物RNA提取試劑盒累計(jì)發(fā)表文章超過200篇。詳細(xì)樣品列表請(qǐng)參考公司主頁(yè)產(chǎn)品介紹或者找我們索取200多篇發(fā)表文章原文。
5.多次柱漂洗確保高純度,OD260/OD280典型的比值達(dá)1.9~2.2,無DNA殘留,可用于熒光定量PCR、RT-PCR、芯片、二代測(cè)序、Northern-blot等各種實(shí)驗(yàn)。
使用EASYspin/EASYspin Plus系列植物RNA提取試劑盒已經(jīng)有大量文章發(fā)表,請(qǐng)聯(lián)系我們索取發(fā)表的文章和包括北京林業(yè)大學(xué),北京農(nóng)學(xué)院,中國(guó)農(nóng)業(yè)大學(xué),農(nóng)林科學(xué)院果樹研究所,林業(yè)所,中國(guó)農(nóng)科院等大學(xué)和研究院所大量使用案例。
1. 桃果實(shí)、花、根、葉:Isolation, characterisation and phylogenetic analysis of resistance gene analogues in a wild species of peach (Prunus kansuensis).Canadian Journal of Plant Science, 2011, 91(6): 961-970
2. 櫻桃花、葉、顎等各部位:Over-expression of the PaAP1 gene from sweet cherry (Prunus avium L.) causes early floweri.Journal of Plant Physiology, 2012,Available online 1 December 2012
3. 洋蔥根、莖、蕾、葉、雌雄蕊等各部位:Cloning and Expression Analysis of A Putative B Class MADS-box Gene of AcPI in Onion. Scientia Agricultura Sinica, 2012, 45(23):4759-4769
4. 蕪菁:Isolation and Functional Characterisation of the Genes Encoding Δ8-Sphingolipid Desaturase from Brassica rapa. Journal of Genetics and Genomics Volume 39, Issue 1, January 2012, Pages 47–59
5. 蕪 菁 1 :EXPRESSION, DIVERGENCE AND EVOLUTION OF THE CALEOSIN GENE FAMILY IN BRASSICA
6. 番茄葉:Effect of Low Temperature Stress on the Expression of ProDH Gene and the Activities of the Proline Dehydrogenase in Leaves of Tomato Seedling. Chinese Agricultural Science Bulletin 2012,28(10):132-135
7. 梔子葉:Isolation of High Quality Total RNA from Gardenia jasminoides Eills.Chinese Agricultural Science Bulletin.2012, 28(27):194-198
8. 油桐果實(shí):Cui Qinqin, Han Xiaojiao, Chen Yicun, Zhan Zhiyong, Lin Liyuan, Wang Yangdong. Isolation and Expression Characteristics of Biotin Carboxyl Carrier Protein Coding Gene(VfBCCP) from Vernicia fordii.SCIENTIA SILVAE SINICAE. 2012, 48(8): Available online August
9. 油桐果實(shí)1:Selection of Reliable Reference Genes for Gene Expression Studies Using Real-Time PCR in Tung Tree during Seed Development. PLoS ONE, 2012, 7(8): e43084
10. 紫菜:Molecular cloning and expression analysis of ribosomal protein S7 gene from Porphyra haitanensis. JOURNAL OF FISHERIES OF CHINA, 2011, 35(12):1814-1821
11. 石斛:Molecular characterization of a mitogen-activated protein kinase gene DoMPK
12. 石斛1:ESTs Analysis Reveals Putative Genes Involved in Symbiotic Seed Germination in Dendrobium officinale. Symbiotic Germination Genes in D. officinale. August 2013 | Volume 8 | Issue 8 | e72705
13. 大豆:RNA-seq Analysis Reveals Ethylene-Mediated Reproductive Organ Development and Abscission in Soybean(Glycine max L. Merr.). Plant Mol Biol Rep, 2012, published online: 4 Dec, 2012
14. 大豆1:Construction of ethylene regulatory network based on the phytohormones related gene transcriptome profiling and prediction of transcription factor activities in soybean. Acta Physiol Plant, 2012, published online: 12 Dec, 2012
15. 紅花玉蘭:Expression Analysis of MAwuAG in Different Organs and Developmental Stages of Magnolia wufengensis. Chinese Bulletin of Botany, 2013, 48 (2): 1–5
16. 毛桃:Cloning and Phylogeny Analysis of PpAP2 Floral Homologous Genes in Peach. Chinese Agricultural Science Bulletin, 2013, 29(7): 99-104
17. 五倍子:Cloning and characterisation of a phenylalanine ammonia-lyase gene from Rhus chinensis. Plant Cell Rep, 2013, published online:15 March, 2013
18. :五倍子1:Cloning, characterization and expression of chalcone synthase from medicinal plant Rhus chinensis.J. Plant Biochem. Biotechnol. DOI 10.1007/s13562-013-0231-9
19. 青杄 :cDNA Cloning and Bioinformatic Analysis of the sPPa1 Gene form Picea wilsonii. Plant Science Journal, 2012, 30(40): 394-401
20. 青杄 1:cDNA Cloning and Bioinformatic Analysis of PsbO Gene from Picea wilsonii.Life Science Research, 2012, 16(3): 201-206
21. 青杄 2:Cloning and Tissue Expression Analysis of PwPSAF in Picea wilsonii. SCIENTIA SILVAE SINICAE. Vol. 49,No. 10, Oct. 2013.
22. 洋蔥:Molecular Cloning and Transcriptional Analysis of the Putative AGAMOUS Homolog AcAG in Onion (Allium cepa. Plant Mol Biol Rep, DOI 10.1007/s11105-013-0607-y
23. 木瓜:XsFAD2 gene encodes the enzyme responsible for the high linoleic acid content in oil accumulated in Xanthoceras sorbifolia seeds. JOURNAL ARTICLE.
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