Thrombin (MW 37kDa)  (Synonyms: 凝血酶)

Thrombin (MW 37kDa) 是一種 Na+ 激huo的胰蛋白酶樣別構(gòu)絲氨酸蛋白酶。Thrombin 能夠利用凝血酶識別序列對融合蛋白進(jìn)行位點(diǎn)特異性切割，并可用于消化 GST 標(biāo)記的蛋白。

生物活性

Thrombin (MW 37kDa) is a Na⁺-activated, allosteric serine protease that plays opposing functional roles in blood coagulation. Thrombin recognition sequence and can be used to digest GST-tagged proteins.

體外研究(In Vitro)

p62 is cleaved from the agarose beads as well as the GST tag by addition of Thrombin (1 Unit/100 μg protein) at room temperature for 20 h^[3].
Thrombin solution: Dissolve in PBS prechilled to 4°C. Swirl gently. Store solution in small aliquots at -80°C to preserve activity.
Cleavage buffer: PBS (140 mM NaCl, 2.7 mM KCl, 10 mM Na₂HPO₄, 1.8 mM KH₂PO₄) pH 7.3.
Elution buffer: 50 mM Tris-HCl, 10 mM reduced glutathione, pH 8.0

分子量:37000(Average)

性狀:Solid

CAS 號:9002-04-4

中文名稱:凝血酶

運(yùn)輸條件

Room temperature in continental US; may vary elsewhere

儲存方式

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

參考文獻(xiàn)

[1]. Golderman V, et al. Thrombin and the Protease-Activated Receptor-1 in Organophosphate-Induced Status Epilepticus. J Mol Neurosci. 2018 Dec 4.  [Content Brief]

[2]. Krenzlin H, et al. The Importance of Thrombin in Cerebral Injury and Disease. Int J Mol Sci. 2016 Jan 11;17(1).  [Content Brief]

[3]. Geetha T, et al. p62 serves as a shuttling factor for TrkA interaction with the proteasome. Biochem Biophys Res Commun. 2008 Sep 12;374(1):33-7.  [Content Brief]