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白蛋白(ALB)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)
編號(hào)CEB028Hu
物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
實(shí)驗(yàn)方法競(jìng)爭(zhēng)抑制
反應(yīng)時(shí)長(zhǎng)2h
檢測(cè)范圍246.9-20,000ng/mL
靈敏度最小可檢測(cè)劑量小于等于89.6ng/mL.
樣本類型Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
規(guī)格48T96T 96T*5 96T*10 96T*100
價(jià)格￥ 1621
特異性
本試劑盒用于檢測(cè)白蛋白(ALB)，經(jīng)檢測(cè)與其它相似物質(zhì)無(wú)明顯交叉反應(yīng)。
由于受到技術(shù)及樣本來(lái)源的限制，不可能完成對(duì)所有相關(guān)或相似物質(zhì)交叉反應(yīng)檢測(cè)，因此本試劑盒有可能與未經(jīng)檢測(cè)的其它物質(zhì)有交叉反應(yīng)。
回收率
分別于定值血清及血漿樣本中加入一定量的白蛋白(ALB)（加標(biāo)樣品），重復(fù)測(cè)定并計(jì)算其均值，回收率為測(cè)定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| serum(n=5) | 88-98 | 94 |
| EDTA plasma(n=5) | 96-103 | 101 |
| heparin plasma(n=5) | 80-96 | 83 |
精密度
精密度用樣品測(cè)定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對(duì)低、中、高值定值樣本進(jìn)行定量檢測(cè),每份樣本連續(xù)測(cè)定20 次，分別計(jì)算不同濃度樣本的平均值及SD值。
批間差：選取3個(gè)不同批次的試劑盒分別對(duì)低、中、高值定值樣本進(jìn)行定量測(cè)定，每個(gè)樣本使用同一試劑盒重復(fù)測(cè)定8次，分別計(jì)算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內(nèi)加入適量的白蛋白(ALB)，并倍比稀釋成1:2，1:4，1:8，1:16的待測(cè)樣本，線性范圍即為稀釋后樣本中白蛋白(ALB)含量的測(cè)定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 81-91% | 80-91% | 92-99% | 86-104% |
| EDTA plasma(n=5) | 86-103% | 78-89% | 94-101% | 82-94% |
| heparin plasma(n=5) | 99-105% | 91-99% | 87-97% | 93-102% |
穩(wěn)定性
經(jīng)測(cè)定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對(duì)試劑盒破壞前后檢測(cè)值的影響，實(shí)驗(yàn)室的環(huán)境條件需盡量保持一致，尤其是實(shí)驗(yàn)室內(nèi)溫度、濕度及溫育條件。其次由同一實(shí)驗(yàn)員來(lái)進(jìn)行操作可減少人為誤差。
實(shí)驗(yàn)流程
1. 實(shí)驗(yàn)前標(biāo)準(zhǔn)品、試劑及樣本的準(zhǔn)備；
2. 加樣（標(biāo)準(zhǔn)品及樣本）50μL，
加入50μL檢測(cè)液A(臨用前配制);
37°C溫育1小時(shí)。
3. 洗板3次；
4. 加檢測(cè)溶液B100μL，37°C孵育30分鐘；
5. 洗板5次；
6. 加TMB底物90μL，37°C孵育10-20分鐘；
7. 加終止液50μL，立即450nm讀數(shù)。
實(shí)驗(yàn)原理
本試劑盒應(yīng)用競(jìng)爭(zhēng)抑制酶聯(lián)免疫分析法測(cè)定標(biāo)本中待測(cè)物質(zhì)水平。將白蛋白(ALB)單克隆抗體包被微孔板，制成固相載體，往包被抗體的微孔中同時(shí)加入生物素標(biāo)記的抗原和待測(cè)抗原(標(biāo)準(zhǔn)品或樣本)，待測(cè)抗原與生物素標(biāo)記抗原對(duì)特異性抗體進(jìn)行競(jìng)爭(zhēng)結(jié)合。溫育后經(jīng)洗滌去掉未結(jié)合物，然后加入HRP標(biāo)記的親和素，經(jīng)過(guò)溫育和徹底洗滌后加入底物TMB顯色。TMB在過(guò)氧化物酶的催化下轉(zhuǎn)化成藍(lán)色，并在酸的作用下轉(zhuǎn)化成最終的黃色。待測(cè)標(biāo)本濃度越高，標(biāo)記抗原和抗體的結(jié)合就越受到抑制，顯色愈淺。顯色的深淺與酶量呈正相關(guān)，而與樣品中待測(cè)物質(zhì)含量呈負(fù)相關(guān)。用酶標(biāo)儀在450nm波長(zhǎng)下測(cè)定吸光度（O.D.值），計(jì)算樣品濃度。
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| PLoS One | Simvastatin Reduces Endotoxin-Induced Acute Lung Injury by Decreasing Neutrophil Recruitment and Radical Formation [PubMed: 22701728] |
| Liver International | Albumin administration protects against bilirubin-induced auditory brainstem dysfunction in Gunn rat pups [Onlinelibrary: liv.12219] |
| Journal of Infectious Diseases | Adjunctive corticosteroid therapy improves lung immunopathology and survival during severe secondary pneumococcal pneumonia in mice [Pubmed: 24273183] |
| Toxins (Basel). | Increased Circulating Levels of Vitamin D Binding Protein in MS Patients [Pubmed:25590278] |
| Mol Cell Biochem | The wnt/β-catenin signaling pathway participates in rhein ameliorating kidney injury in DN mice [PubMed: 26346164] |
| PLoS One | Collecting Protein Biomarkers in Breath Using Electret Filters: A Preliminary Method on New Technical Model and Human Study [Pubmed:26934615] |
| Journal of Biomaterials and Tissue Engineering | 3D Printing of Differentiated Bone Marrow Mesenchymal Cells as a New Method for Liver Tissue Engineering [art00007] |
| Experimental and molecular pathology | Alteration of Connexin43 expression in a rat model of obesity-related glomerulopathy. [pubmed:29246788] |
| Inflammation | Resolvin D1 Promotes SIRT1 Expression to Counteract the Activation of STAT3 and NF-κB in Mice with Septic-Associated Lung Injury [Pubmed:30014231] |
| Journal of Developmental Origins of Health and Disease | Maternal low-quality protein diet exerts sex-specific effects on plasma amino acid profile and alters hepatic expression of methyltransferases in adult rat offspring [Pubmed:29582727] |
| Medicine | Electret filter collects more exhaled albumin than glass condenser: A method comparison based on human study [Pubmed:29384875] |
| Stem Cell Research & Therapy | Therapeutic efficiency of human amniotic epithelial stem cell-derived functional hepatocyte-like cells in mice with acute hepatic failure [Pubmed: 30463600] |
| JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A | Decellularized Human Liver Scaffold‐Based Three‐dimensional Culture System Facilitate HBV Infection [Pubmed: 30963688] |
| biomedicine & pharmacotherapy | Treatment with 3, 4-dihydroxyphenylethyl alcohol glycoside ameliorates sepsis-induced ALI in mice by reducing inflammation and regulating M1 polarization [Pubmed: 31146107] |
| biochemical and biophysical research communications | Anti-proteinuria effect of antibody against ANGPTL3 coil-coiled domain on adriamycin-induced nephropathy in mice [Pubmed: 31256934] |
| Diabetes, Metabolic Syndrome and Obesity | A small molecule inhibitor MCC950 ameliorates kidney injury in diabetic nephropathy by inhibiting NLRP3 inflammasome activation [Pubmed: 31447572] |
| Molecular and Cellular Endocrinology | Diiodothyronines regulate metabolic homeostasis in primary human hepatocytes by modulating mTORC1 and mTORC2 activity [Pubmed: 31580898] |
| BMC Veterinary Research | Preliminary study on the effects of enrofloxacin, flunixin meglumine and pegbovigrastim on Mycoplasma bovis pneumonia [Pubmed: 31655595] |
| International Immunopharmacology | Ghrelin alleviates traumatic brain injury-induced acute lung injury through pyroptosis/NF-κB pathway [Pubmed: 31918060] |
| HYPERTENSION RESEARCH | Sodium butyrate ameliorates deoxycorticosterone acetate/salt-induced hypertension and renal damage by inhibiting the MR/SGK1 pathway [Pubmed: 32908237] |
| Obesity Surgery | Sleeve Gastrectomy Attenuates Diabetic Nephropathy by Upregulating Nephrin Expressions in Diabetic Obese Rats [Pubmed: 32399849] |
| Protective Role of Training and Selenium Consumption Against Renal Toxicity Induced by Cadmium in Rats [] | |
| Nutrition | Gut Dysbiosis Contribute to High Fructose Induced Salt Sensitive Hypertension In Sprague-Dawley Rats [] |
| sensors and actuators b-chemical | A light-induced self-powered competitive immunosensor for the detection of platelet derived growth factor-BB via an elaborately assembled bioconjugate [] |
| Biochem Biophys Res Commun | cGAS promotes sepsis in radiotherapy of cancer by up-regulating caspase-11 signaling [33721834] |
| Journal of Chinese Pharmaceutical Sciences | A simple and sensitive electrochemical impedimetric immunosensor for the detection of apolipoprotein E4 based on CeZnO nanoparticle [] |
| Inflammation | Syringaresinol Resisted Sepsis-Induced Acute Lung Injury by Suppressing Pyroptosis Via the Oestrogen Receptor-β Signalling Pathway [34807349] |
| Nat Commun | GP73 is a TBC-domain Rab GTPase-activating protein contributing to the pathogenesis of non-alcoholic fatty liver disease without obesity [34853313] |
| ACS Appl Mater Interfaces | Emitter–Quencher Pair of Single Atomic Co Sites and Monolayer Titanium Carbide MXenes for Luminol Chemiluminescent Reactions [34914377] |
| Food & Function | Moderation of gut microbiota and bile acid metabolism by chlorogenic acid improves high-fructose-induced salt-sensitive hypertension in mice [Pubmed:35708145] |
| Authorea | Syringaresinol attenuates kidney injury by inhibition of pyroptosis via activating the Nrf2 antioxidant pathway in diabetic nephropathy [] |
白蛋白(ALB)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)
編號(hào)CEB028Hu
物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
實(shí)驗(yàn)方法競(jìng)爭(zhēng)抑制
反應(yīng)時(shí)長(zhǎng)2h
檢測(cè)范圍246.9-20,000ng/mL
靈敏度最小可檢測(cè)劑量小于等于89.6ng/mL.
樣本類型Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
規(guī)格48T96T 96T*5 96T*10 96T*100
價(jià)格￥ 1621
特異性
本試劑盒用于檢測(cè)白蛋白(ALB)，經(jīng)檢測(cè)與其它相似物質(zhì)無(wú)明顯交叉反應(yīng)。
由于受到技術(shù)及樣本來(lái)源的限制，不可能完成對(duì)所有相關(guān)或相似物質(zhì)交叉反應(yīng)檢測(cè)，因此本試劑盒有可能與未經(jīng)檢測(cè)的其它物質(zhì)有交叉反應(yīng)。
回收率
分別于定值血清及血漿樣本中加入一定量的白蛋白(ALB)（加標(biāo)樣品），重復(fù)測(cè)定并計(jì)算其均值，回收率為測(cè)定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| serum(n=5) | 88-98 | 94 |
| EDTA plasma(n=5) | 96-103 | 101 |
| heparin plasma(n=5) | 80-96 | 83 |
精密度
精密度用樣品測(cè)定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對(duì)低、中、高值定值樣本進(jìn)行定量檢測(cè),每份樣本連續(xù)測(cè)定20 次，分別計(jì)算不同濃度樣本的平均值及SD值。
批間差：選取3個(gè)不同批次的試劑盒分別對(duì)低、中、高值定值樣本進(jìn)行定量測(cè)定，每個(gè)樣本使用同一試劑盒重復(fù)測(cè)定8次，分別計(jì)算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內(nèi)加入適量的白蛋白(ALB)，并倍比稀釋成1:2，1:4，1:8，1:16的待測(cè)樣本，線性范圍即為稀釋后樣本中白蛋白(ALB)含量的測(cè)定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 81-91% | 80-91% | 92-99% | 86-104% |
| EDTA plasma(n=5) | 86-103% | 78-89% | 94-101% | 82-94% |
| heparin plasma(n=5) | 99-105% | 91-99% | 87-97% | 93-102% |
穩(wěn)定性
經(jīng)測(cè)定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對(duì)試劑盒破壞前后檢測(cè)值的影響，實(shí)驗(yàn)室的環(huán)境條件需盡量保持一致，尤其是實(shí)驗(yàn)室內(nèi)溫度、濕度及溫育條件。其次由同一實(shí)驗(yàn)員來(lái)進(jìn)行操作可減少人為誤差。
實(shí)驗(yàn)流程
1. 實(shí)驗(yàn)前標(biāo)準(zhǔn)品、試劑及樣本的準(zhǔn)備；
2. 加樣（標(biāo)準(zhǔn)品及樣本）50μL，
加入50μL檢測(cè)液A(臨用前配制);
37°C溫育1小時(shí)。
3. 洗板3次；
4. 加檢測(cè)溶液B100μL，37°C孵育30分鐘；
5. 洗板5次；
6. 加TMB底物90μL，37°C孵育10-20分鐘；
7. 加終止液50μL，立即450nm讀數(shù)。
實(shí)驗(yàn)原理
本試劑盒應(yīng)用競(jìng)爭(zhēng)抑制酶聯(lián)免疫分析法測(cè)定標(biāo)本中待測(cè)物質(zhì)水平。將白蛋白(ALB)單克隆抗體包被微孔板，制成固相載體，往包被抗體的微孔中同時(shí)加入生物素標(biāo)記的抗原和待測(cè)抗原(標(biāo)準(zhǔn)品或樣本)，待測(cè)抗原與生物素標(biāo)記抗原對(duì)特異性抗體進(jìn)行競(jìng)爭(zhēng)結(jié)合。溫育后經(jīng)洗滌去掉未結(jié)合物，然后加入HRP標(biāo)記的親和素，經(jīng)過(guò)溫育和徹底洗滌后加入底物TMB顯色。TMB在過(guò)氧化物酶的催化下轉(zhuǎn)化成藍(lán)色，并在酸的作用下轉(zhuǎn)化成最終的黃色。待測(cè)標(biāo)本濃度越高，標(biāo)記抗原和抗體的結(jié)合就越受到抑制，顯色愈淺。顯色的深淺與酶量呈正相關(guān)，而與樣品中待測(cè)物質(zhì)含量呈負(fù)相關(guān)。用酶標(biāo)儀在450nm波長(zhǎng)下測(cè)定吸光度（O.D.值），計(jì)算樣品濃度。
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| PLoS One | Simvastatin Reduces Endotoxin-Induced Acute Lung Injury by Decreasing Neutrophil Recruitment and Radical Formation [PubMed: 22701728] |
| Liver International | Albumin administration protects against bilirubin-induced auditory brainstem dysfunction in Gunn rat pups [Onlinelibrary: liv.12219] |
| Journal of Infectious Diseases | Adjunctive corticosteroid therapy improves lung immunopathology and survival during severe secondary pneumococcal pneumonia in mice [Pubmed: 24273183] |
| Toxins (Basel). | Increased Circulating Levels of Vitamin D Binding Protein in MS Patients [Pubmed:25590278] |
| Mol Cell Biochem | The wnt/β-catenin signaling pathway participates in rhein ameliorating kidney injury in DN mice [PubMed: 26346164] |
| PLoS One | Collecting Protein Biomarkers in Breath Using Electret Filters: A Preliminary Method on New Technical Model and Human Study [Pubmed:26934615] |
| Journal of Biomaterials and Tissue Engineering | 3D Printing of Differentiated Bone Marrow Mesenchymal Cells as a New Method for Liver Tissue Engineering [art00007] |
| Experimental and molecular pathology | Alteration of Connexin43 expression in a rat model of obesity-related glomerulopathy. [pubmed:29246788] |
| Inflammation | Resolvin D1 Promotes SIRT1 Expression to Counteract the Activation of STAT3 and NF-κB in Mice with Septic-Associated Lung Injury [Pubmed:30014231] |
| Journal of Developmental Origins of Health and Disease | Maternal low-quality protein diet exerts sex-specific effects on plasma amino acid profile and alters hepatic expression of methyltransferases in adult rat offspring [Pubmed:29582727] |
| Medicine | Electret filter collects more exhaled albumin than glass condenser: A method comparison based on human study [Pubmed:29384875] |
| Stem Cell Research & Therapy | Therapeutic efficiency of human amniotic epithelial stem cell-derived functional hepatocyte-like cells in mice with acute hepatic failure [Pubmed: 30463600] |
| JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A | Decellularized Human Liver Scaffold‐Based Three‐dimensional Culture System Facilitate HBV Infection [Pubmed: 30963688] |
| biomedicine & pharmacotherapy | Treatment with 3, 4-dihydroxyphenylethyl alcohol glycoside ameliorates sepsis-induced ALI in mice by reducing inflammation and regulating M1 polarization [Pubmed: 31146107] |
| biochemical and biophysical research communications | Anti-proteinuria effect of antibody against ANGPTL3 coil-coiled domain on adriamycin-induced nephropathy in mice [Pubmed: 31256934] |
| Diabetes, Metabolic Syndrome and Obesity | A small molecule inhibitor MCC950 ameliorates kidney injury in diabetic nephropathy by inhibiting NLRP3 inflammasome activation [Pubmed: 31447572] |
| Molecular and Cellular Endocrinology | Diiodothyronines regulate metabolic homeostasis in primary human hepatocytes by modulating mTORC1 and mTORC2 activity [Pubmed: 31580898] |
| BMC Veterinary Research | Preliminary study on the effects of enrofloxacin, flunixin meglumine and pegbovigrastim on Mycoplasma bovis pneumonia [Pubmed: 31655595] |
| International Immunopharmacology | Ghrelin alleviates traumatic brain injury-induced acute lung injury through pyroptosis/NF-κB pathway [Pubmed: 31918060] |
| HYPERTENSION RESEARCH | Sodium butyrate ameliorates deoxycorticosterone acetate/salt-induced hypertension and renal damage by inhibiting the MR/SGK1 pathway [Pubmed: 32908237] |
| Obesity Surgery | Sleeve Gastrectomy Attenuates Diabetic Nephropathy by Upregulating Nephrin Expressions in Diabetic Obese Rats [Pubmed: 32399849] |
| Protective Role of Training and Selenium Consumption Against Renal Toxicity Induced by Cadmium in Rats [] | |
| Nutrition | Gut Dysbiosis Contribute to High Fructose Induced Salt Sensitive Hypertension In Sprague-Dawley Rats [] |
| sensors and actuators b-chemical | A light-induced self-powered competitive immunosensor for the detection of platelet derived growth factor-BB via an elaborately assembled bioconjugate [] |
| Biochem Biophys Res Commun | cGAS promotes sepsis in radiotherapy of cancer by up-regulating caspase-11 signaling [33721834] |
| Journal of Chinese Pharmaceutical Sciences | A simple and sensitive electrochemical impedimetric immunosensor for the detection of apolipoprotein E4 based on CeZnO nanoparticle [] |
| Inflammation | Syringaresinol Resisted Sepsis-Induced Acute Lung Injury by Suppressing Pyroptosis Via the Oestrogen Receptor-β Signalling Pathway [34807349] |
| Nat Commun | GP73 is a TBC-domain Rab GTPase-activating protein contributing to the pathogenesis of non-alcoholic fatty liver disease without obesity [34853313] |
| ACS Appl Mater Interfaces | Emitter–Quencher Pair of Single Atomic Co Sites and Monolayer Titanium Carbide MXenes for Luminol Chemiluminescent Reactions [34914377] |
| Food & Function | Moderation of gut microbiota and bile acid metabolism by chlorogenic acid improves high-fructose-induced salt-sensitive hypertension in mice [Pubmed:35708145] |
| Authorea | Syringaresinol attenuates kidney injury by inhibition of pyroptosis via activating the Nrf2 antioxidant pathway in diabetic nephropathy [] |
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