取消
清空記錄
歷史記錄
清空記錄
歷史記錄
產(chǎn)品中心
瀏覽器自帶分享功能也很好用哦~
在線客服
客服一
3-Methyladenine (Synonyms: 3-甲基腺嘌呤; 3-MA)
3-Methyladenine (3-MA) 是 PI3K 的抑制劑。它通過(guò)抑制class III PI3K廣作為自噬 (autophagy) 的抑制劑使用。
生物活性
3-Methyladenine (3-MA) is a PI3K inhibitor. 3-Methyladenine is a widely used inhibitor of autophagy via its inhibitory effect on class III PI3K
體外研究(In Vitro)
3-Methyladenine (0-10 mM; 0-48 hours) induces caspase-dependent cell death in HeLa cells in a time-and dose-dependent manner[2].
3-Methyladenine (5 mM; 24 hours) suppresses autophagy in HeLa cells under both glucose-free conditions and normal conditions[2].
3-Methyladenine (5 mM; 0-48 hours) suppresses conversion of LC3-I to LC3-II (autophagy markers) between 12hours and 48 hours, confirms the inhibitory effects on autophagy[2].
3-Methyladenine induces cell death is independent of autophagy inhibition[2].
3-Methyladenine significantly shortens the duration of nocodazole-induced-prometaphase arrest[2].
Note:
在體外細(xì)胞培養(yǎng)實(shí)驗(yàn)中，3-MA 常用的濃度為 0.5-10 mM，所以優(yōu)先推薦直接稱(chēng)取本次實(shí)驗(yàn)所需粉末量，用培養(yǎng)基完全溶解，0.22 μm 過(guò)濾器過(guò)濾除菌之后使用。建議溶液現(xiàn)配現(xiàn)用，盡快用完。
Cell Viability Assay[2]
| Cell Line: | HeLa cells |
| Concentration: | 0 mM, 2.5 mM or 5 mM, 10 mM |
| Incubation Time: | 0 hour, 24 hours and 48 hours |
| Result: | Decreased cell viability in a time-and dose-dependent manner, and was associated with caspase-3 activation. |
體內(nèi)研究(In Vivo)
3-Methyladenine (1.5?mg/100?g; intraperitoneal injection; 3-24?hours) treatment alleviates sodium taurocholate-induced severe acute pancreatitis (SAP) in rats at both 12 hours and 24?hours[3].
3-Methyladenine inhibits autophagy of pancreatic acinar cells in sodium taurocholate-tnduced SAP[3].
3-Methyladenine also shows inhibitory effects on PI3K/Akt signaling pathway and NF-κB signaling pathway in sodium taurocholate-induced SAP[3].
| Animal Model: | 10–12 weeks Specific pathogen free- (SPF-) grade healthy male Sprague-Dawley (SD) rats (250–290?g)[3] |
| Dosage: | 1.5?mg/100?g (1000 μM) |
| Administration: | Intraperitoneal injection |
| Result: | Alleviated Sodium Taurocholate-Induced SAP. |
分子量：149.15
Formula：C6H7N5
CAS 號(hào)：5142-23-4
中文名稱(chēng)：3-甲基腺嘌呤
運(yùn)輸條件：Room temperature in continental US; may vary elsewhere.
儲(chǔ)存方式
| Powder | -20°C | 3 years |
|---|---|---|
| 4°C | 2 years | |
| In solvent | -80°C | 6 months |
| -20°C | 1 month |
溶解性數(shù)據(jù)
H2O : 5 mg/mL (33.52 mM; Need ultrasonic)
DMSO : 8.33 mg/mL (55.85 mM; Need ultrasonic)
| 濃度溶劑體積質(zhì)量 | 1 mg | 5 mg | 10 mg |
|---|
| 1 mM | 6.7047 mL | 33.5233 mL | 67.0466 mL |
| 5 mM | 1.3409 mL | 6.7047 mL | 13.4093 mL |
| 10 mM | 0.6705 mL | 3.3523 mL | 6.7047 mL |
請(qǐng)根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲(chǔ)備液；一旦配成溶液，請(qǐng)分裝保存，避免反復(fù)凍融造成的產(chǎn)品失效。
儲(chǔ)備液的保存方式和期限：-80°C, 6 months; -20°C, 1 month。-80°C 儲(chǔ)存時(shí)，請(qǐng)?jiān)?6 個(gè)月內(nèi)使用，-20°C 儲(chǔ)存時(shí)，請(qǐng)?jiān)?1 個(gè)月內(nèi)使用。
以下溶解方案都請(qǐng)先按照 In Vitro 方式配制澄清的儲(chǔ)備液，再依次添加助溶劑：
——為保證實(shí)驗(yàn)結(jié)果的可靠性，澄清的儲(chǔ)備液可以根據(jù)儲(chǔ)存條件，適當(dāng)保存；體內(nèi)實(shí)驗(yàn)的工作液，建議您現(xiàn)用現(xiàn)配，當(dāng)天使用； 以下溶劑前顯示的百
分比是指該溶劑在您配制終溶液中的體積占比；如在配制過(guò)程中出現(xiàn)沉淀、析出現(xiàn)象，可以通過(guò)加熱和/或超聲的方式助溶
- 1.
請(qǐng)依序添加每種溶劑： PBS
Solubility: 4 mg/mL (26.82 mM); Clear solution; Need ultrasonic
參考文獻(xiàn)
[1]. Miller S, et al. Finding a fitting shoe for Cinderella: searching for an autophagy inhibitor. Autophagy. 2010 Aug;6(6):805-7. Content Brief
[2]. Hou H, et al. Inhibitors of phosphatidylinositol 3'-kinases promote mitotic cell death in HeLa cells. PLoS One. 2012;7(4):e35665. Content Brief
[3]. Wang X, et al. Acanthopanax versus 3-Methyladenine Ameliorates Sodium Taurocholate-Induced Severe Acute Pancreatitis by Inhibiting the Autophagic Pathway in Rats.Mediators Inflamm. 2016;2016:8369704. Content Brief
3-Methyladenine (Synonyms: 3-甲基腺嘌呤; 3-MA)
3-Methyladenine (3-MA) 是 PI3K 的抑制劑。它通過(guò)抑制class III PI3K廣作為自噬 (autophagy) 的抑制劑使用。
生物活性
3-Methyladenine (3-MA) is a PI3K inhibitor. 3-Methyladenine is a widely used inhibitor of autophagy via its inhibitory effect on class III PI3K
體外研究(In Vitro)
3-Methyladenine (0-10 mM; 0-48 hours) induces caspase-dependent cell death in HeLa cells in a time-and dose-dependent manner[2].
3-Methyladenine (5 mM; 24 hours) suppresses autophagy in HeLa cells under both glucose-free conditions and normal conditions[2].
3-Methyladenine (5 mM; 0-48 hours) suppresses conversion of LC3-I to LC3-II (autophagy markers) between 12hours and 48 hours, confirms the inhibitory effects on autophagy[2].
3-Methyladenine induces cell death is independent of autophagy inhibition[2].
3-Methyladenine significantly shortens the duration of nocodazole-induced-prometaphase arrest[2].
Note:
在體外細(xì)胞培養(yǎng)實(shí)驗(yàn)中，3-MA 常用的濃度為 0.5-10 mM，所以優(yōu)先推薦直接稱(chēng)取本次實(shí)驗(yàn)所需粉末量，用培養(yǎng)基完全溶解，0.22 μm 過(guò)濾器過(guò)濾除菌之后使用。建議溶液現(xiàn)配現(xiàn)用，盡快用完。
Cell Viability Assay[2]
| Cell Line: | HeLa cells |
| Concentration: | 0 mM, 2.5 mM or 5 mM, 10 mM |
| Incubation Time: | 0 hour, 24 hours and 48 hours |
| Result: | Decreased cell viability in a time-and dose-dependent manner, and was associated with caspase-3 activation. |
體內(nèi)研究(In Vivo)
3-Methyladenine (1.5?mg/100?g; intraperitoneal injection; 3-24?hours) treatment alleviates sodium taurocholate-induced severe acute pancreatitis (SAP) in rats at both 12 hours and 24?hours[3].
3-Methyladenine inhibits autophagy of pancreatic acinar cells in sodium taurocholate-tnduced SAP[3].
3-Methyladenine also shows inhibitory effects on PI3K/Akt signaling pathway and NF-κB signaling pathway in sodium taurocholate-induced SAP[3].
| Animal Model: | 10–12 weeks Specific pathogen free- (SPF-) grade healthy male Sprague-Dawley (SD) rats (250–290?g)[3] |
| Dosage: | 1.5?mg/100?g (1000 μM) |
| Administration: | Intraperitoneal injection |
| Result: | Alleviated Sodium Taurocholate-Induced SAP. |
分子量：149.15
Formula：C6H7N5
CAS 號(hào)：5142-23-4
中文名稱(chēng)：3-甲基腺嘌呤
運(yùn)輸條件：Room temperature in continental US; may vary elsewhere.
儲(chǔ)存方式
| Powder | -20°C | 3 years |
|---|---|---|
| 4°C | 2 years | |
| In solvent | -80°C | 6 months |
| -20°C | 1 month |
溶解性數(shù)據(jù)
H2O : 5 mg/mL (33.52 mM; Need ultrasonic)
DMSO : 8.33 mg/mL (55.85 mM; Need ultrasonic)
| 濃度溶劑體積質(zhì)量 | 1 mg | 5 mg | 10 mg |
|---|
| 1 mM | 6.7047 mL | 33.5233 mL | 67.0466 mL |
| 5 mM | 1.3409 mL | 6.7047 mL | 13.4093 mL |
| 10 mM | 0.6705 mL | 3.3523 mL | 6.7047 mL |
請(qǐng)根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲(chǔ)備液；一旦配成溶液，請(qǐng)分裝保存，避免反復(fù)凍融造成的產(chǎn)品失效。
儲(chǔ)備液的保存方式和期限：-80°C, 6 months; -20°C, 1 month。-80°C 儲(chǔ)存時(shí)，請(qǐng)?jiān)?6 個(gè)月內(nèi)使用，-20°C 儲(chǔ)存時(shí)，請(qǐng)?jiān)?1 個(gè)月內(nèi)使用。
以下溶解方案都請(qǐng)先按照 In Vitro 方式配制澄清的儲(chǔ)備液，再依次添加助溶劑：
——為保證實(shí)驗(yàn)結(jié)果的可靠性，澄清的儲(chǔ)備液可以根據(jù)儲(chǔ)存條件，適當(dāng)保存；體內(nèi)實(shí)驗(yàn)的工作液，建議您現(xiàn)用現(xiàn)配，當(dāng)天使用； 以下溶劑前顯示的百
分比是指該溶劑在您配制終溶液中的體積占比；如在配制過(guò)程中出現(xiàn)沉淀、析出現(xiàn)象，可以通過(guò)加熱和/或超聲的方式助溶
- 1.
請(qǐng)依序添加每種溶劑： PBS
Solubility: 4 mg/mL (26.82 mM); Clear solution; Need ultrasonic
參考文獻(xiàn)
[1]. Miller S, et al. Finding a fitting shoe for Cinderella: searching for an autophagy inhibitor. Autophagy. 2010 Aug;6(6):805-7. Content Brief
[2]. Hou H, et al. Inhibitors of phosphatidylinositol 3'-kinases promote mitotic cell death in HeLa cells. PLoS One. 2012;7(4):e35665. Content Brief
[3]. Wang X, et al. Acanthopanax versus 3-Methyladenine Ameliorates Sodium Taurocholate-Induced Severe Acute Pancreatitis by Inhibiting the Autophagic Pathway in Rats.Mediators Inflamm. 2016;2016:8369704. Content Brief
取消
清空記錄
歷史記錄
清空記錄
歷史記錄
