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瀏覽器自帶分享功能也很好用哦~型號(hào)：HY-17561
CAS：108321-42-2
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G-418 disulfate (Geneticin sulfate) 是一種氨基糖苷類抗生素，抑制真核生物和原核生物的蛋白質(zhì)合成。G-418 disulfate 是一種常用的真核細(xì)胞選擇性藥劑。
生物活性：
G-418 disulfate (Geneticin sulfate), is an aminoglycoside antibiotic, inhibits protein synthesis in eukaryotes and prokaryotes. G-418 disulfate is commonly used as a selective agent for eukaryotic cells[1].
體外研究(In Vitro)
G418 sulfate, an aminoglycoside neomycin analogue, interferes with protein synthesis and has been used extensively for selection of mammalian cell lines that possess neomycin resistance (NR). The neomycin resistance (neo) gene is frequently used in eukaryotic vectors as a dominant selectable gene. G418 can be covalently bound to asialoorosomucoid (AsOR) to form a conjugate for hepatocyte-specific targeting and toxicity[2].
The human GD3 synthase cDNA was transfected into MDA-MB231 cells, and G-418 bulk selection was used to select cells stably overexpressing the GD3 synthase[2]. An aminoglycoside antibiotic, G418, has been shown to be an inhibitor of many pro- and eukaryotes at concentrations from 1-300 microgram/ml[4].
分子量：692.71
Formula：C20H44N4O18S2
CAS 號(hào)：108321-42-2
中文名稱：遺傳霉素硫酸鹽
結(jié)構(gòu)分類：
來源：Micromonospora rhodorangea
運(yùn)輸條件：Room temperature in continental US; may vary elsewhere.
儲(chǔ)存方式：
4°C, stored under nitrogen, away from moisture
*In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen, away from moisture)
溶解性數(shù)據(jù)
H2O : 125 mg/mL (180.45 mM; Need ultrasonic)
| 濃度溶劑體積質(zhì)量 | 1 mg | 5 mg | 10 mg |
|---|
| 1 mM | 1.4436 mL | 7.2180 mL | 14.4361 mL |
| 5 mM | 0.2887 mL | 1.4436 mL | 2.8872 mL |
| 10 mM | 0.1444 mL | 0.7218 mL | 1.4436 mL |
請(qǐng)根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲(chǔ)備液；一旦配成溶液，請(qǐng)分裝保存，避免反復(fù)凍融造成的產(chǎn)品失效。
儲(chǔ)備液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (stored under nitrogen, away from moisture)。-80°C 儲(chǔ)存時(shí)，請(qǐng)?jiān)?6 個(gè)月內(nèi)使用，-20°C 儲(chǔ)存時(shí)，請(qǐng)?jiān)?1 個(gè)月內(nèi)使用。
以下溶解方案都請(qǐng)先按照 In Vitro 方式配制澄清的儲(chǔ)備液，再依次添加助溶劑：
——為保證實(shí)驗(yàn)結(jié)果的可靠性，澄清的儲(chǔ)備液可以根據(jù)儲(chǔ)存條件，適當(dāng)保存；體內(nèi)實(shí)驗(yàn)的工作液，建議您現(xiàn)用現(xiàn)配，當(dāng)天使用； 以下溶劑前顯示的百
分比是指該溶劑在您配制終溶液中的體積占比；如在配制過程中出現(xiàn)沉淀、析出現(xiàn)象，可以通過加熱和/或超聲的方式助溶
- 1.
請(qǐng)依序添加每種溶劑： PBS
Solubility: 50 mg/mL (72.18 mM); Clear solution; Need ultrasonic
參考文獻(xiàn)
G-418 disulfate (Geneticin sulfate) 是一種氨基糖苷類抗生素，抑制真核生物和原核生物的蛋白質(zhì)合成。G-418 disulfate 是一種常用的真核細(xì)胞選擇性藥劑。
生物活性：
G-418 disulfate (Geneticin sulfate), is an aminoglycoside antibiotic, inhibits protein synthesis in eukaryotes and prokaryotes. G-418 disulfate is commonly used as a selective agent for eukaryotic cells[1].
體外研究(In Vitro)
G418 sulfate, an aminoglycoside neomycin analogue, interferes with protein synthesis and has been used extensively for selection of mammalian cell lines that possess neomycin resistance (NR). The neomycin resistance (neo) gene is frequently used in eukaryotic vectors as a dominant selectable gene. G418 can be covalently bound to asialoorosomucoid (AsOR) to form a conjugate for hepatocyte-specific targeting and toxicity[2].
The human GD3 synthase cDNA was transfected into MDA-MB231 cells, and G-418 bulk selection was used to select cells stably overexpressing the GD3 synthase[2]. An aminoglycoside antibiotic, G418, has been shown to be an inhibitor of many pro- and eukaryotes at concentrations from 1-300 microgram/ml[4].
分子量：692.71
Formula：C20H44N4O18S2
CAS 號(hào)：108321-42-2
中文名稱：遺傳霉素硫酸鹽
結(jié)構(gòu)分類：
來源：Micromonospora rhodorangea
運(yùn)輸條件：Room temperature in continental US; may vary elsewhere.
儲(chǔ)存方式：
4°C, stored under nitrogen, away from moisture
*In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen, away from moisture)
溶解性數(shù)據(jù)
H2O : 125 mg/mL (180.45 mM; Need ultrasonic)
| 濃度溶劑體積質(zhì)量 | 1 mg | 5 mg | 10 mg |
|---|
| 1 mM | 1.4436 mL | 7.2180 mL | 14.4361 mL |
| 5 mM | 0.2887 mL | 1.4436 mL | 2.8872 mL |
| 10 mM | 0.1444 mL | 0.7218 mL | 1.4436 mL |
請(qǐng)根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲(chǔ)備液；一旦配成溶液，請(qǐng)分裝保存，避免反復(fù)凍融造成的產(chǎn)品失效。
儲(chǔ)備液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (stored under nitrogen, away from moisture)。-80°C 儲(chǔ)存時(shí)，請(qǐng)?jiān)?6 個(gè)月內(nèi)使用，-20°C 儲(chǔ)存時(shí)，請(qǐng)?jiān)?1 個(gè)月內(nèi)使用。
以下溶解方案都請(qǐng)先按照 In Vitro 方式配制澄清的儲(chǔ)備液，再依次添加助溶劑：
——為保證實(shí)驗(yàn)結(jié)果的可靠性，澄清的儲(chǔ)備液可以根據(jù)儲(chǔ)存條件，適當(dāng)保存；體內(nèi)實(shí)驗(yàn)的工作液，建議您現(xiàn)用現(xiàn)配，當(dāng)天使用； 以下溶劑前顯示的百
分比是指該溶劑在您配制終溶液中的體積占比；如在配制過程中出現(xiàn)沉淀、析出現(xiàn)象，可以通過加熱和/或超聲的方式助溶
- 1.
請(qǐng)依序添加每種溶劑： PBS
Solubility: 50 mg/mL (72.18 mM); Clear solution; Need ultrasonic
參考文獻(xiàn)
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