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瀏覽器自帶分享功能也很好用哦~
RNA提取試劑
TRIpure Reagent（Trizol總RNA提取試劑）
TRIpure 試劑是直接從細(xì)胞或組織中提取總RNA的試劑。
它在破碎和溶解細(xì)胞時能保持RNA的完整性。加入氯仿后離心，樣品分成水樣層和有機(jī)層。RNA存在于水樣層中。收集上面的水樣層后，RNA可以通過異丙醇沉淀來還原。在除去水樣層后，樣品中的DNA和蛋白也能相繼以沉淀的方式還原。乙醇沉淀能析出中間層的DNA，在有機(jī)層中加入異丙醇能沉淀出蛋白。共純化DNA對于樣品間標(biāo)準(zhǔn)化RNA的產(chǎn)量十分有用。
無論是人、動物、植物還是細(xì)菌組織，該方法對少量的組織(50-100mg)和細(xì)胞(5×106)以及大量的組織(≥1g)和細(xì)胞(>107)均有較好的分離效果。TRIpure 試劑操作上的簡單性允許同時處理多個的樣品。所有的操作可以在一小時內(nèi)完成。TRIpure抽提的總RNA能夠避免DNA和蛋白的污染。故而能夠作RNA印跡分析、斑點雜交、poly(A)+選擇、體外翻譯、RNA酶保護(hù)分析和分子克隆。如果是用于PCR，當(dāng)兩條引物位于單一外顯子內(nèi)時，建議用擴(kuò)增級的DNase I來處理抽提的總RNA。
TRIpure 試劑能促進(jìn)不同種屬不同分子量大小的多種RNA的析出。例如，從大鼠肝臟抽提的RNA瓊脂糖凝膠電泳并用溴化乙啶染色，可見許多介于7 kb和15 kb之間不連續(xù)的高分子量條帶(mRNA和hnRNA成分)，兩條優(yōu)勢核糖體~5 kb (28S)和~2 kb(18S)，低分子量RNA介于0.1和0.3 kb之間 (tRNA, 5S)。當(dāng)抽提的RNA用TE稀釋時其A260/A280比值≥1.8。
性價比優(yōu)之“評委”
“評委”A：100%替代進(jìn)口TRIzol
“評委”B：每次OEM訂單體量之大都需要用托車?yán)?/span>
“評委”C：受到國外眾多小國采購的青睞及非常好評
“評委”D：較多引用文獻(xiàn)
“評委”E：價格極優(yōu)
RN01 TRIpure（TRIzol）總RNA提取試劑盒發(fā)表文章
1. Effect and Molecular Mechanism of DHA on Improving Rats Learning and Memory Ability. Food Science, 2012, online
2. Differential expression of microRNAs in human keloids. Chinese Journal of Tissue Engineering Research, 2012, 16(50): 9370-9375.
3. Study of Multidrug-resistance Reversing of Curcumin and Verapamil on Human Gastric Carcinoma SGC7901/ADR Cells.Vol.34,No.2,Apr.2012,pp.322-325
4. Effect of Sanguisorba tannins on the expression of MGMT gene and protein in myelosuppressed mice. TUMOR Vol. 33, September 2013
5. Zinc oxide nanoparticles inhibit Ca2+-ATPase expression in human lens epithelial cells under UVB irradiation. Toxicology in Vitro 27 (2013) 2117–2126
6. Effects of Du Meridian Electroacupuncture on Growth Associated Protein- 43 Expression in Rats after Spinal Cord Injury. Chin J Rehabil Theory Pract, Jan. 2014, Vol. 20, No.1.
7. Mycobacterium Bovis Ornithine Carbamoyltransferase,MB1684, Induces Proinflammatory Cytokine Gene. Expression by Activating NF-kB in Macrophages. ORIGINAL RESEARCH ARTICLE. DNA AND CELL BIOLOGY. Volume 33, Number 5, 2014a Mary Ann Liebert, Inc.Pp. 1–9. DOI: 10.1089/dna.2013.2026.
8. A Novel Method of Total RNA Extraction from Leaves of.China Biotechnology,2013,33(5):107-111
9. The Response to High Magneto-Gravitational Environment of Different Differentiating-Stage Osteoblasts. Chinese Journal of Cell Biology 2013, 35(7):
10. Disrupted calcium homeostasis is involved in elevated zinc ion-induced photoreceptor cell death. Archives of Biochemistry and Biophysics 2014, 560: 44–51
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